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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00250 [old locus tag: SAUSA300_0047 ]
- pan locus tag?: SAUPAN000800000
- symbol: SAUSA300_RS00250
- pan gene symbol?: —
- synonym:
- product: HNH endonuclease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00250 [old locus tag: SAUSA300_0047 ]
- symbol: SAUSA300_RS00250
- product: HNH endonuclease
- replicon: chromosome
- strand: +
- coordinates: 59098..60063
- length: 966
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (59098..60063) NCBI
- BioCyc: SAUSA300_RS00250 BioCyc
- MicrobesOnline: see SAUSA300_0047
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAGTATAAGCGAATATAAAAATAGATACTTAGAGTCACTATATAACACCTTTAAAACT
GAGTTAGAAAACGAACGCACTCGCTATTTTATATTAGATACCAACTATACAAATAGTGAT
GATACTGAAAAGGAGTTTACTTGGGATGTAAAACAGAATAATAAGATCAGAGAAGGCGAT
TTGTTCATTTTTAGACGGCCAACTAATTTATCTCAAATTGCTCAACAATTTTATTTCTTT
GGAGCTGGGAAAATAGAAAAAATTGAGCGAAAAAATAATGTAGCAACCGCTTACATTTCA
AAACCCTTACTTTTTGTGGATCGTGTTTTAAAAGATAATATTGAAAATTTAAAATGGGAG
TTCAAAGAAAGAATTAAAGAAGAGTGGGAGCAGTTTTTCTTTAAAAATAAAATCAGTAAT
ATTACAAAAAATGATTTTTTAAAACTACTAAGTTTGAGTAGAAATGTAGTGGAAATAGAA
AGTAATTATATTAATAATGATATTTCGCTAACTTTTAAACAAATGGCTGATATGGCAACT
CGAATAAAGAAAAATATTTACTACGTAGGGAATCATGAAGCATTTGTTCAAGCTAAGGGA
AGTGCTCATTTTGAGTTTTCAAAAAGGATTAAACAAAATTATAGTTATAGATGTGCAATT
ACCGGGATTAAAACTAAAGACTTTTTAGTCGTTACACATATTATTCCTTGGCATGAAAAT
GAGTTTATTAGACTAGATCCTTCAAATGGTATTTGTTTGTCTTTATTTTTAGCAAAAGCA
TTTAAGAAAGGCTTTATCACTTTTTCTAACAGTTATAGAGTTGTTTTATCTAAAGAAGCA
GAGAAAGATGCTGCTTTATATGAAGAATTAAAGATTTATGAAAATCAAAAAATCGAACTA
CCAGATTGTCAGAAACCTAATTTGAAATATTTAGATTGGCATAGAGAACATATTTTTAAA
AATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00250 [old locus tag: SAUSA300_0047 ]
- symbol: SAUSA300_RS00250
- description: HNH endonuclease
- length: 321
- theoretical pI: 9.33864
- theoretical MW: 38374.5
- GRAVY: -0.603738
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SAUSA300_0047
- PFAM: His-Me_finger (CL0263) HNH_2; HNH endonuclease (PF13391; HMM-score: 26.8)and 2 moreHNH; HNH endonuclease (PF01844; HMM-score: 14.6)Actin_ATPase (CL0108) FtsA; Cell division protein FtsA (PF14450; HMM-score: 12.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005872
- TAT(Tat/SPI): 0.000309
- LIPO(Sec/SPII): 0.000913
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 445950117 NCBI
- RefSeq: WP_000027972 NCBI
- UniProt: see SAUSA300_0047
⊟Protein sequence[edit | edit source]
- MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEKEFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKPLLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIESNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRCAITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVLSKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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