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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
  • pan locus tag?: SAUPAN000813000
  • symbol: SAUSA300_RS00325
  • pan gene symbol?: arcD
  • synonym:
  • product: arginine-ornithine antiporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
  • symbol: SAUSA300_RS00325
  • product: arginine-ornithine antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 71606..73027
  • length: 1422
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATGAAAATAAATTAGGTAAAACTTCCTTAATTGGTTTAGTCATAGGCTCTATGATA
    GGCGGTGGTGCATTCAATATCATCTCAGATATGGGTGGCCAAGCTGGTGGACTTGCAATA
    ATTATCGGTTGGATAATAACTGCTATTGGTATGATTTCTCTTGCTTTCGTATTTCAAAAT
    TTAACAAATGAGCGACCAGATCTTGATGGAGGAATTTATAGTTATGCTCAAACAGGGTTT
    GGAGATTTTATTGGTTTTTCAAGTGCTTGGGGATATTGGTTTGCAGCATTTCTAGGTAAT
    GTGGCTTATGCAACCCTATTAATGTCAGCTGTGGGTAACTTTTTCCCTATATTTAAAGGA
    GGTAACACACTTCCAAGTATTATCATAGCATCAATTTTATTATGGGGTGTACATTTTTTA
    ATACTTAGAGGTGTAGAAACTGCAGCGTTTATAAATAGTATTGTTACAGTAGCTAAATTA
    ATACCTATATTTCTAGTTATTATATGCATGATAGTTGTATTCAACTTCAGTACTTTTAAA
    TCCGGTTTTTATGGTATGACTAGTGGAAGTGTTGGCGTTTTTAGTTGGGGAGATACAATG
    GCACAAGTAAAAAGTACTATGTTAGTAACTGTATGGGTATTCACAGGGATTGAAGGAGCC
    GTTGTCTTTTCTGGACGTGCAAAGTCTAAAAAGGATGTAGGAACTGCTACCGTTATTGGT
    TTGATTTCTGTGCTAGTCATTTATTTCTTAATGACTGTACTAGCCCAAGGTGTCATTCAG
    CAGAACCAAATTTCAAAACTTGCTAATCCATCAATGGCACAAGTATTAGAACATATTGTA
    GGTCATTGGGGTTCAGTGTTAGTTAATATAGGCTTAATTATCTCTGTTTTAGGAGCTTGG
    TTAGGATGGACATTACTAGCTGGTGAATTACCATTCATTGTAGCTAAAGATGGACTTTTC
    CCGAAATGGTTTGCTAAAGAAAATAAGAATAAAGCTCCGGTCAACGCTTTAATTATTACT
    AATATATTAGTTCAGTTATTTTTAATTAGTATGTTGTTTACAGATAGTGCCTATCAGTTT
    GCGTTTTCACTTGCATCAAGTGCAATCTTAATTCCATATACACTCAGTGCTTTTTACCAG
    GTTAAATATACTATTCAAAATAAATCTAAAGCTAATTTAAAACAATGGATAATAGGAATT
    ATTGCATCTATTTACACAATTTGGTTGGTTTATGCAGCTGGATTAGATTATTTACTATTA
    ACGATGTTGTTATATATACCTGGATTACTCGTATACAGCTACGTACAAAGGGATAATAAC
    AAACATTTGACAAAATTGGATTATACGTTATTCATATTCATCATTGTACTTGCAATAATA
    GGAATAGTTCGTTTGATTACAGGTAATATTTCTGTATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1422

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
  • symbol: SAUSA300_RS00325
  • description: arginine-ornithine antiporter
  • length: 473
  • theoretical pI: 9.58322
  • theoretical MW: 51409.6
  • GRAVY: 0.873784

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 641.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 585.6)
    and 8 more
    histidine-histamine antiporter (TIGR04298; HMM-score: 144.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 99.8)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 86.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 54.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 53.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 43.2)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 30.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 25.3)
  • TheSEED: see SAUSA300_0064
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 253.1)
    and 3 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 86)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 23.3)
    no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 10.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011535
    • TAT(Tat/SPI): 0.000572
    • LIPO(Sec/SPII): 0.014888
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGWIITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKLIPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIEGAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVLEHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPVNALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKANLKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTLFIFIIVLAIIGIVRLITGNISVF

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]