PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
• pan locus tag^?: SAUPAN000813000
• symbol: SAUSA300_RS00325
• pan gene symbol^?: arcD
• synonym:
• product: arginine-ornithine antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
• symbol: SAUSA300_RS00325
• product: arginine-ornithine antiporter
• replicon: chromosome
• strand: -
• coordinates: 71606..73027
• length: 1422
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (71606..73027) NCBI
• BioCyc: SAUSA300_RS00325 BioCyc
• MicrobesOnline: see SAUSA300_0064

⊟Phenotype

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⊟DNA sequence

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  ATGGATGAAAATAAATTAGGTAAAACTTCCTTAATTGGTTTAGTCATAGGCTCTATGATA
  GGCGGTGGTGCATTCAATATCATCTCAGATATGGGTGGCCAAGCTGGTGGACTTGCAATA
  ATTATCGGTTGGATAATAACTGCTATTGGTATGATTTCTCTTGCTTTCGTATTTCAAAAT
  TTAACAAATGAGCGACCAGATCTTGATGGAGGAATTTATAGTTATGCTCAAACAGGGTTT
  GGAGATTTTATTGGTTTTTCAAGTGCTTGGGGATATTGGTTTGCAGCATTTCTAGGTAAT
  GTGGCTTATGCAACCCTATTAATGTCAGCTGTGGGTAACTTTTTCCCTATATTTAAAGGA
  GGTAACACACTTCCAAGTATTATCATAGCATCAATTTTATTATGGGGTGTACATTTTTTA
  ATACTTAGAGGTGTAGAAACTGCAGCGTTTATAAATAGTATTGTTACAGTAGCTAAATTA
  ATACCTATATTTCTAGTTATTATATGCATGATAGTTGTATTCAACTTCAGTACTTTTAAA
  TCCGGTTTTTATGGTATGACTAGTGGAAGTGTTGGCGTTTTTAGTTGGGGAGATACAATG
  GCACAAGTAAAAAGTACTATGTTAGTAACTGTATGGGTATTCACAGGGATTGAAGGAGCC
  GTTGTCTTTTCTGGACGTGCAAAGTCTAAAAAGGATGTAGGAACTGCTACCGTTATTGGT
  TTGATTTCTGTGCTAGTCATTTATTTCTTAATGACTGTACTAGCCCAAGGTGTCATTCAG
  CAGAACCAAATTTCAAAACTTGCTAATCCATCAATGGCACAAGTATTAGAACATATTGTA
  GGTCATTGGGGTTCAGTGTTAGTTAATATAGGCTTAATTATCTCTGTTTTAGGAGCTTGG
  TTAGGATGGACATTACTAGCTGGTGAATTACCATTCATTGTAGCTAAAGATGGACTTTTC
  CCGAAATGGTTTGCTAAAGAAAATAAGAATAAAGCTCCGGTCAACGCTTTAATTATTACT
  AATATATTAGTTCAGTTATTTTTAATTAGTATGTTGTTTACAGATAGTGCCTATCAGTTT
  GCGTTTTCACTTGCATCAAGTGCAATCTTAATTCCATATACACTCAGTGCTTTTTACCAG
  GTTAAATATACTATTCAAAATAAATCTAAAGCTAATTTAAAACAATGGATAATAGGAATT
  ATTGCATCTATTTACACAATTTGGTTGGTTTATGCAGCTGGATTAGATTATTTACTATTA
  ACGATGTTGTTATATATACCTGGATTACTCGTATACAGCTACGTACAAAGGGATAATAAC
  AAACATTTGACAAAATTGGATTATACGTTATTCATATTCATCATTGTACTTGCAATAATA
  GGAATAGTTCGTTTGATTACAGGTAATATTTCTGTATTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS00325 [old locus tag: SAUSA300_0064 ]
• symbol: SAUSA300_RS00325
• description: arginine-ornithine antiporter
• length: 473
• theoretical pI: 9.58322
• theoretical MW: 51409.6
• GRAVY: 0.873784

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 641.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 585.6)
  and 8 more
  histidine-histamine antiporter (TIGR04298; HMM-score: 144.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 99.8)
  putrescine-ornithine antiporter (TIGR04299; HMM-score: 86.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 54.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 53.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 43.2)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 30.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 25.3)
• TheSEED: see SAUSA300_0064
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 253.1)
  and 3 more
  AA_permease; Amino acid permease (PF00324; HMM-score: 86)
  Spore_permease; Spore germination protein (PF03845; HMM-score: 23.3)
  no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 10.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 13
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011535
  • TAT(Tat/SPI): 0.000572
  • LIPO(Sec/SPII): 0.014888
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 446266287 NCBI
• RefSeq: WP_000344142 NCBI
• UniProt: see SAUSA300_0064

⊟Protein sequence

• MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGWIITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKLIPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIEGAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVLEHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPVNALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKANLKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTLFIFIIVLAIIGIVRLITGNISVF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: ArgR/AhrC see SAUSA300_0064

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications