PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
• pan locus tag^?: SAUPAN000821000
• symbol: SAUSA300_RS00370
• pan gene symbol^?: —
• synonym:
• product: nickel ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
• symbol: SAUSA300_RS00370
• product: nickel ABC transporter substrate-binding protein
• replicon: chromosome
• strand: +
• coordinates: 80394..81947
• length: 1554
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (80394..81947) NCBI
• BioCyc: SAUSA300_RS00370 BioCyc
• MicrobesOnline: see SAUSA300_0073

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAGGAAATGTGTATTAATGACGGTAGCAGCAAGTGCTACGCTCTTATTGGCAGGTTGT
  GGCAATGGTCAAAAAGAAGATAAAGATGTTACGGTATCGCTACCTACTGAAGCAAAGGCG
  GATAAACTTGACGCGCAAGGCTATGATGCAGCGATGCCCGTTTATAGTGCAGTGTATGAT
  GCATTAGTTAAATATGATAAAGATAAGGGTATTAAAGCAGGTTTAGCAGATAAATGGAGC
  GTTGATGAATCAGGGAAAGTTTATGAATTCCATTTGAAAAAGAATGTTAAATTCTCAGAT
  GGTTCAGCATTAGATGCTAAGGACGTGAAATTCTCGATTGATCGTGCGAAAGCGATGAAC
  AAAGATTCGACTGTAGAAACGTTAAAAAAATTAGATAAGGTCGTTGTTAAAAATGAGCAC
  GTGGTCCAAATTAGATTGAAATCTCCTTCAAATCAAGTGTTAAATGAATTAACACAAGTG
  AGACCGTTGCGTATTATGAGTCCACATTCAGTAGAAGATGGTAAAGTAAACGGTAAATTT
  GAAAAAGCGATTGGAACAGGTGCATTTGTTGTTGATAAAACTGGTAAAGAAAAAACGACA
  ATGAAGCCAAATAAATATTTTGACAACGGTCACCCAGTCAATTATCATCTTGCATTCCAA
  ACGATTGAAGATGGGGACTCAAGAAATTCTGCAGTACAAAGTGGTTCTGTAGATATTTCT
  GGTGGTGCTTTAGGTATGCTCTCAGACGAACAAATCAAACAAGATAAGAAAAATAAGAAC
  TTAACGATTGAAGATAGACCTAGCACAGTAAGTCACTTTATGGCATTTAACCCTAAAAAT
  GATGTATTAAATCAACGCACAATTCGTGAAGCGATAAGTAAGAGCATCGATGCGAAAGAC
  ATTGCGGGCAAATCTGTAAATGGTCTGTTCCAGAAGAACGTACAATTTGTGACTAAAAAT
  AATCAACAGCCACACGATTATGATATGAAAGCGGCTGAAAGGTTACTTAAATCAGAAGGA
  TATCATAAAAACGATGACGGCATCTTTGAAAAGAATGGCAAACCTTTATCATTTAACTTA
  GTCATTCAAACTGCAGAGTTCCCAAATTGGAAAGATAAAGCTGAAAAAGTGCAACGTCAG
  CTTAAACAAGCCGGTATTAAGTTAAATGTGAAAACGTTAGATTCACAATCATACTATGAT
  ACATTATGGACGAAAAAAGACTATGATTTGATTTTCTATAGAACGTATTCAGATGCATTA
  ATGCCTTACAACTTTATAAGTTCAGTGTTTAAAAATAATGATGGTCAACCAGGGGTGTTA
  GCTGATGATGAAACATTAACGAAACAGCTAGACGATTTCCCATCAACCGTATCAAAAGAA
  GACCAACAGTGTTCATTTGATGACATATTTAAACACTTTAATCAACAATACTATGGTGTG
  CCAATTGCTTATCCAAATGAGACGTTTGTAGTGAGTGATAAAGTAAAACAATTCAAATTC
  TCTGGACTTACGGATGCACCAATTGATTATAAAGCGTTGAAAGTTAATGAATAG
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  1554
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
• symbol: SAUSA300_RS00370
• description: nickel ABC transporter substrate-binding protein
• length: 517
• theoretical pI: 8.58302
• theoretical MW: 57943.1
• GRAVY: -0.62824

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 230.6)
  and 1 more
  Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 94.8)
• TheSEED: see SAUSA300_0073
• PFAM:
  PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 232.6)
  and 1 more
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.21
  • Extracellular Score: 0.78
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000344
  • TAT(Tat/SPI): 0.000043
  • LIPO(Sec/SPII): 0.999509
  • Cleavage Site: CS pos: 19-20. LAG-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447146920 NCBI
• RefSeq: WP_001224176 NCBI
• UniProt: see SAUSA300_0073

⊟Protein sequence

• MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLDAQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGSALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQVRPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLAFQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAFNPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAERLLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKTLDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQLDDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPIDYKALKVNE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications