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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS01445 [old locus tag: SAUSA300_0269 ]
  • pan locus tag?: SAUPAN001162000
  • symbol: SAUSA300_RS01445
  • pan gene symbol?: —
  • synonym:
  • product: choloylglycine hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS01445 [old locus tag: SAUSA300_0269 ]
  • symbol: SAUSA300_RS01445
  • product: choloylglycine hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 321025..322017
  • length: 993
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTGCACAGGATTCACAATACAAACTTTAAATAATCAAGTACTTCTTGGACGCACGATG
    GATTATGATTATCCATTAGATGGTTCGCCAGCAGTGACGCCTAGAAATTATCGTTGGAAA
    TCTCGCACTGGCACGACAGGCCAAACGCAATATGGCTTTATTGGCACAGGAACAGATATG
    GAAGGTTTTATTTATGGTGATGGTGTTAATGAACATGGCGTTGCCATTTCAACACAATAT
    TTCCGAGGTTATAGTTCATATGGATCAACACACAAAGCGGACGCGATGAATATTACGCAA
    AATGAAATTGTGACATGGATTTTGGGATATACAACAAGCATTGAAGATATGAAACAACAA
    GCATCCCAAATACATGTTGTAGCTGTATATTTAAATGACATCGGTGAAGTTCCGCCATTG
    CATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAA
    GTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCAT
    TATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTG
    GAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGA
    TTTACTTCAACTGAGCGCTTTGTGAGAATGGCATTTATGAAAGCAAACATTGCTCAAAAC
    AATGATAAAGAAATGGATTTAATGAATGCATTTTATTTATTAGATGCGGTAAATATACCG
    ATTGGAATTGTACGTCCGCATGATGCTGACAATCACTATACGATGTATCAGACCGTAATA
    AATTTAACTACAAGAACGTTATATATTAAGTATTATGGCAGCAATGAATTAGTAGCATTA
    AAGCTCACAGATGATTTAATTAATAGAAAAGATATGACGATTTTTAAGCCTGAGAAGCAT
    ATCACTATTAGAAAGTTGAATGACAATCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS01445 [old locus tag: SAUSA300_0269 ]
  • symbol: SAUSA300_RS01445
  • description: choloylglycine hydrolase
  • length: 330
  • theoretical pI: 5.73897
  • theoretical MW: 37101.5
  • GRAVY: -0.348182

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.24?  ExPASy
    Choloylglycine hydrolase 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H2O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
  • TIGRFAM:
  • TheSEED: see SAUSA300_0269
  • PFAM:
    NTN (CL0052) CBAH; Linear amide C-N hydrolases, choloylglycine hydrolase family (PF02275; HMM-score: 245.1)
    and 1 more
    AAT; Acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase (PF03417; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.072772
    • TAT(Tat/SPI): 0.001134
    • LIPO(Sec/SPII): 0.008375
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTGTTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNEIVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGEVVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLPGGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMYQTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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