COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01595 [old locus tag: SAUSA300_0299 ]
• pan locus tag^?:
• symbol: SAUSA300_RS01595
• pan gene symbol^?: —
• synonym:
• product: TIGR01741 family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01595 [old locus tag: SAUSA300_0299 ]
• symbol: SAUSA300_RS01595
• product: TIGR01741 family protein
• replicon: chromosome
• strand: +
• coordinates: 350651..351151
• length: 501
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (350651..351151) NCBI
• BioCyc: SAUSA300_RS01595 BioCyc
• MicrobesOnline: see SAUSA300_0299

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGACTTTCGAAGAGAAACTAAGTCAAATGTACAATAAAATTGCAAGTGAGATCAGTGGA
  ATGATACCAGTAGAATGGGAGCAAGTATTTACAATAGCTTATGTAACTGATCAAGCTGGG
  GAAGTCATTTTTAATTATACTAAACCAGGTAGTGATGAATTAAATTATTATTCAGACATA
  CCTAAAGATTGCAATGTCTCAAAAGATATTTTTAAGAATTCATGGTTTAAAGTTTATCGA
  ATGTTTGATGAGTTAAGAGAAACTTTTAAAGAAGAAGAGCTTGAACCATGGACATCATGC
  GAATTTGACTTTACAAGAGATGGCAAATTGAATGTATCTTTTGATTATATTGATTGGGTG
  AATTCAGAATTTGGACCAATGGGAAGAGAACATTATTATATGTATAAAAAATTTGGAATT
  TGGCCTGAAAAAGAATATGCCATAAATTGGGTTGAAAAAATAAAAGATTATGTTAAAGAG
  CAAGATGAAGCTGAACTATAG
  60
  120
  180
  240
  300
  360
  420
  480
  501
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS01595 [old locus tag: SAUSA300_0299 ]
• symbol: SAUSA300_RS01595
• description: TIGR01741 family protein
• length: 166
• theoretical pI: 4.19689
• theoretical MW: 20015.3
• GRAVY: -0.68494

⊟Function

• TIGRFAM:
  conserved hypothetical protein (TIGR01741; HMM-score: 255.1)
• TheSEED: see SAUSA300_0299
• PFAM:
  no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 163.4)
  and 2 more
  Holin-II (CL0563) Phage_holin_2_2; Phage holin T7 family, holin superfamily II (PF10746; HMM-score: 13.8)
  no clan defined DUF1031; Protein of unknown function (DUF1031) (PF06275; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008852
  • TAT(Tat/SPI): 0.000555
  • LIPO(Sec/SPII): 0.000768
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446064290 NCBI
• RefSeq: WP_000142145 NCBI
• UniProt: see SAUSA300_0299

⊟Protein sequence

• MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIFNYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKEQDEAEL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications