PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01970 [old locus tag: SAUSA300_0370 ]
• pan locus tag^?: SAUPAN001967000
• symbol: SAUSA300_RS01970
• pan gene symbol^?: selX
• synonym:
• product: toxin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01970 [old locus tag: SAUSA300_0370 ]
• symbol: SAUSA300_RS01970
• product: toxin
• replicon: chromosome
• strand: +
• coordinates: 422037..422648
• length: 612
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (422037..422648) NCBI
• BioCyc: SAUSA300_RS01970 BioCyc
• MicrobesOnline: see SAUSA300_0370

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTCAAAAAATATGACTCAAAAAATTCAATCGTATTAAAATCTATTCTATCGCTAGGT
  ATCATCTATGGGGGAACATTTGGAATATATCCAAAAGCAGACGCGTCAACACAAAATTCC
  TCAAGTGTACAAGATAAACAATTACAAAAAGTTGAAGAAGTACCAAATAATTCAGAAAAA
  GCTTTGGTTAAAAAACTTTACGATAGATACAGCAAGGATACAATAAATGGAAAATCTAAT
  AAATCTAGGAATTGGGTTTATTCAGAGAGACCTTTAAATGAAAACCAAGTTCGTATACAT
  TTAGAAGGAACATACACAGTTGCTGGCAGAGTGTATACACCTAAGAGGAATATTACTCTT
  AATAAAGAAGTTGTCACTTTAAAAGAATTGGATCATATCATAAGATTTGCTCATATTTCC
  TATGGCTTGTATATGGGAGAACATTTGCCTAAAGGTAACATCGTCATAAATACAAAAGAT
  GGTGGTAAATATACATTAGAGTCGCATAAAGAGCTACAAAAAGATAGGGAAAATGTAAAA
  ATTAATACAGCCGATATAAAAAATGTAACTTTCAAACTTGTGAAAAGTGTTAATGACATT
  GAACAAGTTTGA
  60
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  612
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS01970 [old locus tag: SAUSA300_0370 ]
• symbol: SAUSA300_RS01970
• description: toxin
• length: 203
• theoretical pI: 10.0535
• theoretical MW: 23165.2
• GRAVY: -0.656158

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0370
• PFAM:
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 21.4)
  and 2 more
  no clan defined EAR; Ethylene-responsive binding factor-associated repression (PF07897; HMM-score: 12.8)
  Ubiquitin (CL0072) CHIPS; Chemotaxis-inhibiting protein CHIPS (PF11434; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 35 aa
  • SP(Sec/SPI): 0.819885
  • TAT(Tat/SPI): 0.029227
  • LIPO(Sec/SPII): 0.013965
  • Cleavage Site: CS pos: 35-36. ADA-ST. Pr: 0.7459
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446397471 NCBI
• RefSeq: WP_000475326 NCBI
• UniProt: see SAUSA300_0370

⊟Protein sequence

• MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQDKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLEGTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKDGGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0370

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Gillian J Wilson, Keun Seok Seo, Robyn A Cartwright, Timothy Connelley, Olivia N Chuang-Smith, Joseph A Merriman, Caitriona M Guinane, Joo Youn Park, Gregory A Bohach, Patrick M Schlievert, W Ivan Morrison, J Ross Fitzgerald
A novel core genome-encoded superantigen contributes to lethality of community-associated MRSA necrotizing pneumonia.
PLoS Pathog: 2011, 7(10);e1002271
[PubMed:22022262] [WorldCat.org] [DOI] (I p)