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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02000 [old locus tag: SAUSA300_0375 ]
  • pan locus tag?: SAUPAN001975000
  • symbol: SAUSA300_RS02000
  • pan gene symbol?:
  • synonym:
  • product: histidine phosphatase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02000 [old locus tag: SAUSA300_0375 ]
  • symbol: SAUSA300_RS02000
  • product: histidine phosphatase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 425516..426097
  • length: 582
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGATTTATTTAGTTAGACATGGCGAATCAAAATCGAATTATGATAATAAACATTTT
    CGATCTTATTTTTGTGGACAATTAGATGTGCCGTTAACGGATACTGGCACAAAAAGTGCA
    GACGATTTATGTGATTATTTTAAAGAGAAACAGATTAAACATGTATATGTTTCAGACTTA
    TTAAGAACACAGCAAACGTTTGAACATATTTTTCCATATGACATTGCATCAACGACTACC
    CCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGT
    GCGAATCCGAAATATGAAAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGT
    TTTTCACAAAAAGCGCCTGAAGGAGAAAGTTATGAAGATGTATATCAACGCGTAGAACAT
    TTTATGAATCATGTTGTCAATGAAGATACACAAAAAGATGATATTGTCATTGTTGCACAT
    CAAGTTGTCATTCGTTGTTTGATGGTTTATTTTAACAAAGTTTCAAGGGCAGAAGCTGTG
    GATTTAAAGGTAGAAAATTGCAAACCATATATCATTGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    582

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS02000 [old locus tag: SAUSA300_0375 ]
  • symbol: SAUSA300_RS02000
  • description: histidine phosphatase family protein
  • length: 193
  • theoretical pI: 5.57611
  • theoretical MW: 22733.3
  • GRAVY: -0.637824

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin alpha-ribazole phosphatase (TIGR03162; EC 3.1.3.73; HMM-score: 115)
    and 3 more
    phosphoglycerate mutase 1 family (TIGR01258; HMM-score: 60.6)
    probable phosphomutase, MSMEG_4193 family (TIGR03848; EC 5.4.2.-; HMM-score: 36.2)
    Signal transduction Regulatory functions Protein interactions phosphohistidine phosphatase SixA (TIGR00249; EC 3.1.3.-; HMM-score: 25.9)
  • TheSEED: see SAUSA300_0375
  • PFAM:
    His_phosphatase (CL0071) His_Phos_1; Histidine phosphatase superfamily (branch 1) (PF00300; HMM-score: 139.2)
    and 1 more
    no clan defined UNC45-central; Myosin-binding striated muscle assembly central (PF11701; HMM-score: 13.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006877
    • TAT(Tat/SPI): 0.000229
    • LIPO(Sec/SPII): 0.001457
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLCDYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISANPKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAHQVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]