PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS02150 [old locus tag: SAUSA300_0401 ]
• pan locus tag^?: SAUPAN002113000
• symbol: SAUSA300_RS02150
• pan gene symbol^?: ssl7
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS02150 [old locus tag: SAUSA300_0401 ]
• symbol: SAUSA300_RS02150
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 453368..454063
• length: 696
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (453368..454063) NCBI
• BioCyc: SAUSA300_RS02150 BioCyc
• MicrobesOnline: see SAUSA300_0401

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGAAATTAAAAACGTTAGCTAAAGCAACATTGGCATTAGGCTTATTAACTACTGGTGTG
  ATTACATCAGAAGGCCAAGCAGTTCAAGCAAAAGAAAAGCAAGAGAGAGTACAACATTTA
  TATGATATTAAAGACTTACATCGATACTACTCATCAGAAAGTTTTGAATTCAGTAATATT
  AGTGGTAAGGTTGAAAATTATAACGGTTCTAACGTTGTACGCTTTAACCAAGAAAATCAA
  AATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGC
  AAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGT
  GGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAA
  GTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTT
  TCACTGAAAGAACTTGATTTCAAAATTAGACAACATTTAGTTAAAAATTATGGTTTATAT
  AAAGGTACGACTAAATACGGTAAGATCACTATCAATTTGAAAGATGGAGAAAAGCAAGAA
  ATTGATTTAGGTGATAAATTGCAATTCGAGCGCATGGGTGATGTGTTGAATAGTAAGGAT
  ATTAATAAGATTGAAGTGACTTTGAAACAAATTTAA
  60
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  696
  

⊟Protein

• 3KM9
  PDB

Protein Data Bank: 3KM9

⊟General

• locus tag: SAUSA300_RS02150 [old locus tag: SAUSA300_0401 ]
• symbol: SAUSA300_RS02150
• description: hypothetical protein
• length: 231
• theoretical pI: 9.48067
• theoretical MW: 26167.5
• GRAVY: -0.649784

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0401
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 107.9)
  and 2 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 38.5)
  MAP; MAP domain (PF03642; HMM-score: 22.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.98519
  • TAT(Tat/SPI): 0.002363
  • LIPO(Sec/SPII): 0.00867
  • Cleavage Site: CS pos: 30-31. VQA-KE. Pr: 0.8054
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446692490 NCBI
• RefSeq: WP_000769836 NCBI
• UniProt: see SAUSA300_0401

⊟Protein sequence

• MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIKDLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKDVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEVSLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNSKDINKIEVTLKQI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0401

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)