PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS02160 [old locus tag: SAUSA300_0403 ]
• pan locus tag^?: SAUPAN002118000
• symbol: SAUSA300_RS02160
• pan gene symbol^?: ssl9
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS02160 [old locus tag: SAUSA300_0403 ]
• symbol: SAUSA300_RS02160
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 455485..456183
• length: 699
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (455485..456183) NCBI
• BioCyc: SAUSA300_RS02160 BioCyc
• MicrobesOnline: see SAUSA300_0403

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTAACAACGATAGCTAAAGCAACATTAGCATTAGGAATATTAACTACAGGTGTG
  TTTACAGCAGAAAGTCAAACTGGTCACGCGAAAGTAGAACTTGATGAGACACAACGCAAA
  TATTATATCAATATGCTACATCAATACTATTCTGAAGAAAGTTTTGAACCAACAAACATT
  AGTGTTAAAAGCGAAGATTACTATGGCTCTAACGTTTTAAACTTTAAACAACGAAATAAA
  GCTTTTAAAGTATTTTTACTTGGTGACGATAAAAATAAATATAAAGAAAAAACACATGGC
  CTTGATGTCTTTGCAGTACCTGAATTAATAGATATAAAAGGTGGCATATATAGCGTTGGC
  GGTATAACAAAGAAAAATGTGAGATCAGTGTTTGGATTTGTAAGTAATCCAAGTCTACAA
  GTTAAAAAAGTTGATGCTAAAAATGGCTTTTCGATAAACGAGTTGTTTTTTATTCAAAAG
  GAAGAAGTATCATTGAAGGAACTGGACTTTAAAATAAGAAAACTCTTAATCGAAAAATAT
  AGATTGTATAAAGGAACGTCTGATAAAGGTAGAATTGTTATCAATATGAAAGACGAAAAG
  AAGCATGAAATTGATTTAAGTGAAAAATTAAGTTTTGAACGTATGTTTGATGTAATGGAT
  AGTAAGCAAATTAAAAATATTGAAGTGAATTTGAATTAG
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⊟Protein

• 3R2I
  PDB

Protein Data Bank: 3R2I

⊟General

• locus tag: SAUSA300_RS02160 [old locus tag: SAUSA300_0403 ]
• symbol: SAUSA300_RS02160
• description: hypothetical protein
• length: 232
• theoretical pI: 9.69341
• theoretical MW: 26675.5
• GRAVY: -0.450431

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0403
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 109.3)
  and 3 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 39.6)
  MAP; MAP domain (PF03642; HMM-score: 14.3)
  no clan defined MRP-S33; Mitochondrial ribosomal subunit S27 (PF08293; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.922105
  • TAT(Tat/SPI): 0.003753
  • LIPO(Sec/SPII): 0.064595
  • Cleavage Site: CS pos: 30-31. GHA-KV. Pr: 0.5279
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446702100 NCBI
• RefSeq: WP_000779446 NCBI
• UniProt: see SAUSA300_0403

⊟Protein sequence

• MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYINMLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLDVFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQKEEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDVMDSKQIKNIEVNLN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0403

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)