PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS02615 [old locus tag: SAUSA300_0487 ]
• pan locus tag^?: SAUPAN002250000
• symbol: SAUSA300_RS02615
• pan gene symbol^?: tilS
• synonym:
• product: tRNA(Ile)-lysidine synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS02615 [old locus tag: SAUSA300_0487 ]
• symbol: SAUSA300_RS02615
• product: tRNA(Ile)-lysidine synthetase
• replicon: chromosome
• strand: +
• coordinates: 545368..546663
• length: 1296
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (545368..546663) NCBI
• BioCyc: SAUSA300_RS02615 BioCyc
• MicrobesOnline: see SAUSA300_0487

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCAGTTAAATAGTAATGGTTGGCATGTTGATGACCATATTGTTGTCGCTGTTTCTACA
  GGTATTGATAGTATGTGTTTATTGTATCAACTACTAAATGATTATAAAGATAGTTATAGA
  AAACTAACATGCTTACATGTCAATCATGGCGTTAGGTCAGCTTCAATTGAGGAAGCCAGA
  TTTTTAGAAGCATACTGCGAACGTCATCACATCGATTTACATATCAAAAAGTTAGATTTG
  TCGCATAGTCTCGACCGAAATAACAGCATTCAGAATGAAGCTCGAATTAAACGTTACGAA
  TGGTTTGATGAAATGATGAATGTATTAGAAGCGGATGTATTGCTAACGGCGCATCATTTG
  GACGATCAATTAGAAACTATTATGTATCGTATTTTTAATGGGAAATCAACACGTAATAAA
  CTAGGATTTGATGAGTTATCGAAGCGAAAAGGTTATCAGATTTATCGACCACTTTTAGCT
  GTCTCTAAAAAAGAAATAAAACAATTCCAAGAGAGATATCATATTCCATATTTTGAAGAT
  GAATCTAATAAAGATAACAAATATGTTAGAAATGATATTCGTAATAGAATTATTCCAGCT
  ATTGATGAAAATAATCAACTTAAAGTATCGCATTTATTAAAATTAAAACAATGGCATGAT
  GAACAATATGATATTTTGCAATATTCAGCTAAACAATTTATTCAAGAATTTGTGAAGTTT
  GATGAACAGTCAAAATATTTAGAGGTTTCTAGACAAGCTTTTAATAACTTACCAAACTCA
  TTAAAGATGGTTGTGTTGGACTGCCTATTATCAAAGTATTATGAGTTGTTTAATATTAGT
  GCTAAAACATACGAAGAGTGGTTTAAACAATTTAGTAGTAAGAAAGCACAATTCAGTATT
  AATCTCACGGATAAATGGATAATTCAAATCGCATATGGTAAATTAATAATAATGGCTAAA
  AATAATGGCGATACATATTTTAGAGTTCAAACAATTAAAAAGCCAGGTAATTATATTTTT
  AACAAATATCGATTAGAGATACATTCTAATTTACCAAAATGTTTATTTCCGCTTACAGTG
  AGAACACGACAAAGTGGCGATACATTTAAACTGAATGGGCGCGATGGTTATAAGAAAGTG
  AATCGCCTGTTTATAGATTGTAAAGTGCCACAGTGGGTTCGGGATCAAATGCCAATCGTA
  TTGGATAAACAACAGCGCATTATTGCGGTAGGAGATTTATATCAACAACAAACAATAAAA
  AAATGGATTATAATTAGTAAAAATGGAGATGAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS02615 [old locus tag: SAUSA300_0487 ]
• symbol: SAUSA300_RS02615
• description: tRNA(Ile)-lysidine synthetase
• length: 431
• theoretical pI: 9.35158
• theoretical MW: 51291.4
• GRAVY: -0.588863

⊟Function

• reaction:
  EC 6.3.4.19?  ExPASy
  tRNA(Ile)-lysidine synthetase (tRNA(Ile2))-cytidine³⁴ + L-lysine + ATP = (tRNA(Ile2))-lysidine³⁴ + AMP + diphosphate + H₂O
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 197.1)
  and 3 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase, C-terminal domain (TIGR02433; EC 6.-.-.-; HMM-score: 71.6)
  Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 13.5)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 12.7)
• TheSEED: see SAUSA300_0487
• PFAM:
  HUP (CL0039) ATP_bind_3; PP-loop family (PF01171; HMM-score: 201.4)
  and 3 more
  PheT-TilS (CL0383) TilS_C; TilS substrate C-terminal domain (PF11734; HMM-score: 50.5)
  HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 22.2)
  PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 21)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002522
  • TAT(Tat/SPI): 0.00021
  • LIPO(Sec/SPII): 0.00101
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447099459 NCBI
• RefSeq: WP_001176715 NCBI
• UniProt: see SAUSA300_0487

⊟Protein sequence

• MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications