PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03705 [old locus tag: SAUSA300_0690 ]
• pan locus tag^?: SAUPAN002590000
• symbol: SAUSA300_RS03705
• pan gene symbol^?: saeS
• synonym:
• product: two-component sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03705 [old locus tag: SAUSA300_0690 ]
• symbol: SAUSA300_RS03705
• product: two-component sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 766186..767241
• length: 1056
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (766186..767241) NCBI
• BioCyc: SAUSA300_RS03705 BioCyc
• MicrobesOnline: see SAUSA300_0690

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGGTGTTATCAATTAGAAGTCAAATCATTATTGGCGTCGTATCGAGTATACTATTAACT
  TCAACTATTTTAGCAATTGCATATATTTTAATGTGGTTTAACGGCCATATGACACTAACT
  TTGACCTTAACGACAATAATTACAAGCTGTTTAACCTTATTAATATGTAGTATTTTTATT
  AATCCACTTATACAAAAAATTAAGCAGTTTAATATAAAAACTAAGCAATTTGCTAACGGA
  AATTACGCAAGCAATGATAAAACGTTTAATTCACCAAAAGAAATTTATGAATTAAATCAA
  TCTTTTAATAAAATGGCTTCTGAAATTACGCAACAAATGAATCAAATTAAATCCGAACAA
  CAAGAAAAAACAGAACTGATTCAAAACTTAGCCCATGATTTAAAAACACCTTTAGCAAGC
  ATTATTTCATATTCTGAAGGACTACGTGATGGTATAATCACTAAGGATCATGAGATTAAA
  GAGTCATACGACATATTAATTAAACAAGCAAACAGATTATCAACATTATTTGATGATATG
  ACTCATATTATCACTTTAAATACAGGTAAAACATATCCCCCAGAATTAATACAACTAGAC
  CAATTACTTGTATCAATATTGCAACCATATGAGCAACGTATCAAACATGAAAACCGCACA
  TTAGAAGTGAATTTCTGTAACGAAATTGATGCATTTTATCAATATCGAACGCCACTTGAG
  CGTATTTTAACAAACTTACTTGATAATGCGCTAAAATTTTCAAATGTTGGTAGTCGCATT
  GATATTAATATTAGTGAAAACGAAGATCAAGATACTATCGACATTGCTATTAGCGATGAA
  GGTATTGGCATTATACCAGAACTACAAGAACGTATATTCGAACGTACATTCAGAGTAGAA
  AACTCTCGTAATACAAAAACGGGTGGTTCTGGATTAGGCTTATATATAGCTAATGAACTC
  GCGCAACAAAATAACGCAAAAATCAGTGTAAGCAGTGATATAGATGTAGGAACTACGATG
  ACTGTAACATTACACAAATTAGACATTACGTCATAA
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  1056
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS03705 [old locus tag: SAUSA300_0690 ]
• symbol: SAUSA300_RS03705
• description: two-component sensor histidine kinase
• length: 351
• theoretical pI: 4.97145
• theoretical MW: 39741.2
• GRAVY: -0.132764

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  EC 2.7.3.-?  ExPASy
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 140.7)
  and 7 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 106.6)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.9)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 64)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
• TheSEED: see SAUSA300_0690
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 78.3)
  and 10 more
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 46.3)
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 20.3)
  HATPase_c_5; GHKL domain (PF14501; HMM-score: 19.6)
  no clan defined HAMP; HAMP domain (PF00672; HMM-score: 18.5)
  HIN; HIN-200/IF120x domain (PF02760; HMM-score: 17.8)
  DUF2969; Protein of unknown function (DUF2969) (PF11184; HMM-score: 15.2)
  Kinetocho_Slk19; Central kinetochore-associated (PF12709; HMM-score: 14.4)
  EI24; Etoposide-induced protein 2.4 (EI24) (PF07264; HMM-score: 14.2)
  DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 13.5)
  Peptidase_M48_N; CAAX prenyl protease N-terminal, five membrane helices (PF16491; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003245
  • TAT(Tat/SPI): 0.000385
  • LIPO(Sec/SPII): 0.016327
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446166557 NCBI
• RefSeq: WP_000244412 NCBI
• UniProt: see SAUSA300_0690

⊟Protein sequence

• MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIAISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0690

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications