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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS04670 [old locus tag: SAUSA300_0865 ]
- pan locus tag?: SAUPAN003070000
- symbol: SAUSA300_RS04670
- pan gene symbol?: pgi
- synonym:
- product: glucose-6-phosphate isomerase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS04670 [old locus tag: SAUSA300_0865 ]
- symbol: SAUSA300_RS04670
- product: glucose-6-phosphate isomerase
- replicon: chromosome
- strand: +
- coordinates: 944997..946328
- length: 1332
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (944997..946328) NCBI
- BioCyc: SAUSA300_RS04670 BioCyc
- MicrobesOnline: see SAUSA300_0865
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGACTCATATTCAATTAGATTTTAGTAAAACGTTAGAATTTTTCGGTGAACACGAATTA
AAACAACAACAAGAAATTGTTAAATCAATTCACAAAACAATTCATGAAGGTACTGGTGCA
GGTAGTGACTTCTTAGGCTGGGTTGATTTACCAGTTGATTACGACAAAGAAGAATTTTCA
AGAATTGTTGAAGCATCAAAACGCATTAAAGAAAATTCTGATGTTTTAGTAGTCATCGGT
ATTGGTGGTTCTTACTTAGGTGCACGTGCAGCAATCGAAATGTTAACGTCATCATTTAGA
AACAGCAATGAATACCCTGAAATTGTATTTGTTGGTAATCACTTATCATCAACATATACG
AAAGAGTTAGTTGATTATTTAGCAGACAAAGATTTCTCTGTAAACGTTATTTCTAAATCT
GGTACAACTACAGAACCAGCAGTTGCATTTAGATTGTTCAAACAATTAGTTGAAGAAAGA
TACGGTAAAGAAGAAGCACAAAAACGTATATTTGCAACAACGGATAAAGAAAAAGGTGCT
TTAAAACAGTTGGCTACAAACGAAGGTTATGAAACGTTTATCGTACCTGATGATGTAGGT
GGAAGATATTCTGTTTTAACAGCAGTAGGATTATTACCAATTGCAACAGCTGGAATTAAC
ATCGAAGCTATGATGATTGGTGCTGCAAAAGCACGTGAAGAATTATCTTCAGATAAATTA
GAAGAAAACATTGCATACCAATATGCGACAATTCGAAACATTTTATATGCAAAAGGTTAT
ACAACAGAAATGTTGATTAACTATGAACCATCTATGCAATACTTTAATGAATGGTGGAAA
CAATTATTTGGTGAATCAGAAGGTAAAGACTTCAAAGGTATCTATCCTTCAAGTGCCAAC
TACACAACTGATTTACATTCTTTAGGTCAATATGTACAAGAAGGCCGTCGCTTCTTATTC
GAAACAGTTGTAAAAGTAAATCATCCTAAATATGATATTACTATTGAAAAAGATAGTGAT
GATCTAGACGGATTAAATTATTTAGCTGGTAAAACAATCGACGAAGTTAACACAAAAGCA
TTCGAAGGTACATTATTAGCGCATACTGATGGTGGTGTTCCTAACATGGTAGTGAACATT
CCACAATTAGATGAAGAAACTTTCGGTTACGTCGTATACTTCTTCGAACTTGCTTGTGCA
ATGAGTGGATACCAATTAGGCGTAAATCCATTTAACCAACCTGGTGTAGAAGCATATAAA
CAAAACATGTTCGCATTATTAGGTAAACCTGGTTTTGAAGACTTGAAAAAAGAATTAGAA
GAACGTTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS04670 [old locus tag: SAUSA300_0865 ]
- symbol: SAUSA300_RS04670
- description: glucose-6-phosphate isomerase
- length: 443
- theoretical pI: 4.54735
- theoretical MW: 49807.8
- GRAVY: -0.325959
⊟Function[edit | edit source]
- reaction: EC 5.3.1.9? ExPASyGlucose-6-phosphate isomerase D-glucose 6-phosphate = D-fructose 6-phosphate
- TIGRFAM: bifunctional phosphoglucose/phosphomannose isomerase (TIGR02128; EC 5.3.1.9; HMM-score: 25.6)and 1 moredihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 13.8)
- TheSEED: see SAUSA300_0865
- PFAM: SIS (CL0067) PGI; Phosphoglucose isomerase (PF00342; HMM-score: 82)and 3 moreno clan defined ELMO_CED12; ELMO/CED-12 family (PF04727; HMM-score: 14.9)GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.6)EDD (CL0245) EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00213
- TAT(Tat/SPI): 0.000403
- LIPO(Sec/SPII): 0.000235
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446071000 NCBI
- RefSeq: WP_000148855 NCBI
- UniProt: see SAUSA300_0865
⊟Protein sequence[edit | edit source]
- MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDFLGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNSNEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEERYGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAGINIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFNEWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDITIEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVVYFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS03530 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_RS06135 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_RS08865 50S ribosomal protein L20 [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS12135 50S ribosomal protein L2 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)