PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS04895 [old locus tag: SAUSA300_0910 ]
• pan locus tag^?: SAUPAN003180000
• symbol: SAUSA300_RS04895
• pan gene symbol^?: mgtE
• synonym:
• product: magnesium transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS04895 [old locus tag: SAUSA300_0910 ]
• symbol: SAUSA300_RS04895
• product: magnesium transporter
• replicon: chromosome
• strand: +
• coordinates: 996651..998036
• length: 1386
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (996651..998036) NCBI
• BioCyc: SAUSA300_RS04895 BioCyc
• MicrobesOnline: see SAUSA300_0910

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  481
  541
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  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGTCAATGAACACAGATGAAAAAGAGCGTGTTCAAGAGGAATTATACGACCAAACTTTA
  TTAGATCAATATTTAGAAAATGATGATATTGATCAATTTAGAGATGAATTTCTAGCATTA
  CACACATATGAACAAAGTGAGTATTTTGAAGATACTACCGATGAAAATAGACAAAAGATT
  TTTCAATATTTATCACCTGAAGAAGTTGCAAATTTCTTTGATCAATTAGATATTGATGAC
  GATGAATATGAGTTGCTATTTGATAAGATGAATGCGACATACGCAAGTCACATATTAGAA
  GAAATGTCATACGACAATGCAGTAGATATTTTAAATGAGTTGACTAAACCAAAAGTTGCT
  AGTCTTTTAACATTGATGAATAAAGATGACGCGAATGAAATCAAAGCATTACTTCACTAT
  GATGAGGATACGGCCGGCGGTATTATGACGACGGAGTATTTATCACTTAAAGCGCATACG
  CCTGTTAAAGAAGCATTATTATTGGTCAAAGCGCAAGCACCAGACGCAGAAACAATATAT
  GTTATATTTGTCGTTGATGATGATGGTAAATTAGTAGGTGTTTTATCGCTAAGAGATTTA
  ATTGTAGCTGAAAATGATGCTTATATTGAAGATATTATGAATGAACGTGTCATTAGTGTG
  AATGTAGCAGACGACCAAGAAGATGTTGCTCAAGTTATGAGAGACTATGATTTCATGGCT
  GTACCTGTTATAGATTACCAAGAACATTTGCTTGGTATCATCACGATTGATGATATTTTA
  GACGTTATGGATGAAGAGGCTAGTGAAGACTACTCTCGTTTAGCCGGGGTATCAGATATC
  GATTCGACTAATGATTCAATCATTAAAACAGCATTAAAACGTTTACCATGGTTGATTATT
  TTAACATTTTTAGGAATGATTACTGCGACAATTTTAGGGAGATTCGAAAAAACATTAGAA
  AATGTAGCGCTACTCGCAGCGTTTATTCCTATTATTAGTGGTATGTCAGGAAATTCAGGT
  ACACAATCTTTAGCCGTTTCAGTTCGTAACATTACGACAGGGGAAATTAATGAGCAAAGT
  AAATTTAGAATTGCATTAAGAGAAGCAGGAAGTGGTGTATTATCGGGTGTTGTATGTTCA
  ACAATATTATTTACAATTATTGTTGCAATATATCATCAGCCACTTTTAGCATTAATCGTT
  GCAGGAAGTTTAACTTGTGCGATGACGGTGGGGACGTTTGTAGGTTCGATGATTCCATTA
  TTGATGAATAAATTAAATATCGATCCAGCAGTGGCTAGTGGACCATTTATTACAACAATT
  AATGATATTATTAGTATGTTGATTTATTTTGGTTTAGCTACATCATTTATGGCTTACTTA
  ATTTAA
  60
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  1386
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS04895 [old locus tag: SAUSA300_0910 ]
• symbol: SAUSA300_RS04895
• description: magnesium transporter
• length: 461
• theoretical pI: 3.87276
• theoretical MW: 51435.2
• GRAVY: 0.13731

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 405.1)
  and 6 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 39.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 32.2)
  Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 18.5)
  glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 15.6)
  Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 13.5)
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 12.7)
• TheSEED: see SAUSA300_0910
• PFAM:
  no clan defined MgtE; Divalent cation transporter (PF01769; HMM-score: 97.2)
  FliG (CL0436) MgtE_N; MgtE intracellular N domain (PF03448; HMM-score: 84.8)
  and 1 more
  no clan defined CBS; CBS domain (PF00571; HMM-score: 72)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002358
  • TAT(Tat/SPI): 0.00418
  • LIPO(Sec/SPII): 0.000491
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 445984562 NCBI
• RefSeq: WP_000062417 NCBI
• UniProt: see SAUSA300_0910

⊟Protein sequence

• MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFGLATSFMAYLI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications