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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS05830 [old locus tag: SAUSA300_1075 ]
- pan locus tag?: SAUPAN003451000
- symbol: SAUSA300_RS05830
- pan gene symbol?: pbp1
- synonym:
- product: penicillin-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS05830 [old locus tag: SAUSA300_1075 ]
- symbol: SAUSA300_RS05830
- product: penicillin-binding protein
- replicon: chromosome
- strand: +
- coordinates: 1173821..1176055
- length: 2235
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1173821..1176055) NCBI
- BioCyc: SAUSA300_RS05830 BioCyc
- MicrobesOnline: see SAUSA300_1075
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2221ATGGCGAAGCAAAAAATTAAAATTAAAAAAAATAAAATAGGGGCAGTCCTACTTGTTGGT
TTATTCGGACTGCTCTTTTTTATATTGGTTTTAAGAATTTCATATATCATGATTACTGGA
CATTCTAATGGTCAAGATTTAGTCATGAAGGCAAATGAAAAGTATTTAGTTAAGAATGCA
CAACAACCAGAACGAGGAAAGATATATGATCGTAATGGTAAAGTGCTAGCAGAAGATGTA
GAAAGATATAAACTTGTTGCAGTAATAGATAAAAAGGCGAGTGCCAATTCTAAAAAACCT
AGGCATGTAGTTGATAAAAAAGAGACTGCAAAGAAATTATCTACAGTCATTAATATGAAG
CCAGAGGAAATTGAAAAGAGACTTAGTCAAAAGAAAGCTTTCCAAATTGAATTTGGACGC
AAAGGAACAAATTTAACGTATCAGGACAAATTGAAAATAGAGAAAATGAATTTGCCTGGT
ATTTCTTTATTGCCTGAAACAGAACGCTTTTATCCAAATGGCAATTTTGCATCACACTTA
ATTGGTAGAGCTCAGAAAAATCCGGATACTGGTGAACTTAAAGGTGCACTTGGAGTTGAA
AAGATTTTTGATAGTTATTTAAGTGGATCTAAAGGATCATTGAGATATATTCATGATATT
TGGGGATATATCGCACCAAATACTAAAAAAGAGAAGCAGCCTAAACGTGGTGATGATGTC
CATTTAACAATCGATTCAAATATTCAAGTATTTGTTGAAGAAGCTTTAGATGGCATGGTT
GAAAGATACCAGCCGAAAGATTTATTTGCGGTTGTCATGGATGCCAAAACTGGAGAAATT
TTAGCATACAGTCAGCGACCAACATTTAATCCTGAAACTGGTAAAGACTTTGGTAAAAAG
TGGGCAAATGACCTTTATCAAAACACATACGAGCCTGGATCAACATTTAAATCATATGGG
TTAGCAGCTGCTATTCAAGAAGGTGCTTTTGATCCTGATAAGAAATATAAATCTGGACAT
AGAGATATTATGGGTTCACGTATTTCAGACTGGAATAGAGTCGGTTGGGGTGAAATCCCA
ATGTCACTCGGATTTACTTATTCATCTAATACATTGATGATGCATTTACAAGATTTAGTT
GGTGCAGACAAAATGAAATCTTGGTATGAACGATTTGGATTTGGAAAATCAACTAAAGGT
ATGTTTGATGGAGAAGCACCTGGTCAAATTGGATGGAGTAATGAGTTGCAACAAAAAACG
TCATCATTTGGTCAATCGACAACAGTAACACCTGTTCAAATGTTACAAGCGCAATCAGCG
TTCTTTAATGATGGTAATATGTTAAAACCATGGTTTGTGAATAGCGTTGAAAATCCTGTT
AGTAAAAGACAATTTTATAAAGGGCAAAAACAAATCGCAGGCAAACCAATAACAAAAGAT
ACTGCTGAAAAAGTTGAAAAGCAATTGGATTTAGTTGTGAATAGTAAGAAGAGTCACGCT
GCAAACTATCGTATTGATGGTTATGAGGTCGAAGGTAAGACTGGTACAGCACAAGTCGCT
GCACCTAATGGTGGTGGATACGTTAAAGGTCCAAACCCATATTTTGTAAGTTTTATGGGT
GACGCGCCGAAGAAAAATCCTAAAGTTATTGTATACGCTGGTATGAGCTTGGCACAAAAA
AATGACCAAGAAGCTTATGAATTAGGTGTTAGTAAAGCGTTTAAACCAATAATGGAAAAT
ACTTTGAAATATTTAAATGTAGGTAAATCAAAAGATGACACATCTAATGCAGAGTATAGT
AAAGTGCCAGATGTTGAAGGTCAAGACAAACAAAAAGCTATTGATAATGTGAGTGCAAAA
TCATTAGAACCAGTTACTATTGGTTCTGGCACACAAATAAAAGCACAATCTATAAAAGCA
GGGAATAAAGTCTTACCTCATAGTAAAGTACTGTTATTAACAGATGGAGACTTAACTATG
CCTGACATGTCAGGATGGACGAAAGAAGATGTCATTGCTTTTGAAAACCTAACAAATATT
AAAGTAAATTTAAAAGGTAGCGGTTTTGTGTCCCACCAATCAATTAGTAAGGGACAAAAA
CTTACTGAAAAAGATAAAATAGACGTAGAATTTTCATCAGAGAATGTAGACAGCAATTCG
ACGAATAATTCTGATTCAAATTCAGATGATAAGAAGAAATCTGACAGTAAAACTGACAAG
GATAAGTCGGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS05830 [old locus tag: SAUSA300_1075 ]
- symbol: SAUSA300_RS05830
- description: penicillin-binding protein
- length: 744
- theoretical pI: 9.86118
- theoretical MW: 82708.4
- GRAVY: -0.643011
⊟Function[edit | edit source]
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 374.7)Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 374.7)and 3 moreCell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 260.1)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 84.8)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 18.1)
- TheSEED: see SAUSA300_1075
- PFAM: Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 277.1)and 3 moreno clan defined PBP_dimer; Penicillin-binding Protein dimerisation domain (PF03717; HMM-score: 81.9)PASTA; PASTA domain (PF03793; HMM-score: 65.1)DdrB; DdrB-like protein (PF12747; HMM-score: 13.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.17
- Cytoplasmic Membrane Score: 9.51
- Cellwall Score: 0.16
- Extracellular Score: 0.15
- Internal Helices: 2
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.07004
- TAT(Tat/SPI): 0.000531
- LIPO(Sec/SPII): 0.004824
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
- GI: 447041407 NCBI
- RefSeq: WP_001118663 NCBI
- UniProt: see SAUSA300_1075
⊟Protein sequence[edit | edit source]
- MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDKDKSD
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_RS02800 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_RS02810 50S ribosomal protein L7/L12 [1] (data from MRSA252) SAUSA300_RS02845 elongation factor G [1] (data from MRSA252) SAUSA300_RS07035 cold-shock protein CspA [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS08730 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS09015 universal stress protein [1] (data from MRSA252) SAUSA300_RS09090 30S ribosomal protein S4 [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12105 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_RS12115 50S ribosomal protein L16 [1] (data from MRSA252) SAUSA300_RS12125 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_RS12130 30S ribosomal protein S19 [1] (data from MRSA252) SAUSA300_RS12135 50S ribosomal protein L2 [1] (data from MRSA252) SAUSA300_RS12145 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_RS12150 50S ribosomal protein L3 [1] (data from MRSA252) SAUSA300_RS13045 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [1] (data from MRSA252) SAUSA300_RS13795 hydroxymethylglutaryl-CoA synthase [1] (data from MRSA252) SAUSA300_RS14105 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)