PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS06390 [old locus tag: SAUSA300_1182 ]
• pan locus tag^?: SAUPAN003592000
• symbol: SAUSA300_RS06390
• pan gene symbol^?: —
• synonym:
• product: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS06390 [old locus tag: SAUSA300_1182 ]
• symbol: SAUSA300_RS06390
• product: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha
• replicon: chromosome
• strand: +
• coordinates: 1300772..1302532
• length: 1761
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1300772..1302532) NCBI
• BioCyc: SAUSA300_RS06390 BioCyc
• MicrobesOnline: see SAUSA300_1182

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAACCACAATTATCGTGGAAAGTTGGCGGTCAACAAGGCGAAGGTATTGAATCAACT
  GGGGAAATCTTCGCTACGGCTATGAATAGAAAAGGATATTATTTATATGGATATAGACAT
  TTTTCAAGTCGTATCAAAGGTGGACATACGAATAATAAAATTAGAGTTTCTACGACGCCT
  GTTCATGCAATTAGTGATGATTTAGATATTTTGATTGCATTTGACCAAGAAACAATTGAT
  GTTAACCATCATGAAATGAGAGAAGACAGTATTATTTTAGCTGATGCCAAGGCTAAACCT
  GTGAAACCAGAAGGATGTCATGCACAGCTTATTGAATTACCTTTTACAGCAACCGCTAAA
  GAATTAGGTACAGCATTAATGAAAAACATGGTTGCAATAGGTGCTACTAGCGCATTGATG
  AATTTGAATACAAATACATTTGAAGAACTTATTACTAATATGTTTTCTAAAAAAGGTGAC
  AAGGTAGTTGAAGTCAATATCCAAGCATTAAACGAAGGTTATCAATTAATGCAATCTCGC
  TTACCTGAAATCTACGGGGACTTTGAATTAGAGTCAACAGATGCACTACCACATCTATAT
  ATGATTGGTAACGATGCCATTGGATTAGGTGCAATTGCTGCAGGTTCACAATTTATGGCG
  GCATATCCTATTACACCTGCGTCTGAAGTTATGGAATATATGATTGCCAATATATCTAAA
  GTAAACGGAGCGGTTATTCAAACAGAAGATGAAATTGCTGCTGTAACTATGGCTATTGGT
  GCAAATTATGGTGGAGTCAGAGCGTTTACGGCTAGTGCTGGTCCAGGTTTATCTTTAATG
  ATGGAAGCAATTGGATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAA
  CGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATG
  ATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGAT
  GCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATA
  GTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAAT
  CGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGAT
  AAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCC
  GGTGTTAAAGGAGGTATTCATCATATAACTGGTGTGGAACACAATGAAGAAGGTAAACCT
  AGTGAATCTGCGTCAAATAGACAACAACAAATGGAAAAACGAATGCGTAAAATTGAGCAG
  TTACTAATTGAATCGCCAGTAGAAGCTAACTTACAACATGAGGATGCAGATATTCTTTAT
  ATCGGTTTTATTTCTACAAAAGGTGCAATTCAAGAAGGTAGTAACCGTTTGAATCAACAA
  GGCATAAAAGTTAACACTATACAAATTAGACAATTGCATCCATTCCCAACAAGCGTTATT
  CAAGATGCAGTTAATAAAGCGAAGAAAGTCGTTGTAGTGGAGCACAATTATCAAGGACAA
  TTGGCTAGTATTATAAAAATGAATGTCAATATTCATGATAAGATTGAAAATTATACAAAG
  TATGATGGGACACCTTTCCTACCACATGAAATCGAAGAAAAAGGCAAAATAATTGCTACT
  GAAATAAAGGAGATGGTATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS06390 [old locus tag: SAUSA300_1182 ]
• symbol: SAUSA300_RS06390
• description: 2-oxoglutarate ferredoxin oxidoreductase subunit alpha
• length: 586
• theoretical pI: 5.06399
• theoretical MW: 64555.1
• GRAVY: -0.276621

⊟Function

• reaction:
  EC 1.2.7.3?  ExPASy
  2-oxoglutarate synthase 2-oxoglutarate + CoA + 2 oxidized ferredoxin = succinyl-CoA + CO₂ + 2 reduced ferredoxin + 2 H⁺
• TIGRFAM:
  2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 712.6)
  and 9 more
  indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 89.1)
  2-oxoacid:acceptor oxidoreductase, gamma subunit, pyruvate/2-ketoisovalerate family (TIGR02175; HMM-score: 37.1)
  pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 25.3)
  indolepyruvate ferredoxin oxidoreductase, beta subunit (TIGR03334; EC 1.2.7.8; HMM-score: 25.2)
  Metabolism Amino acid biosynthesis Aromatic amino acid family 3-dehydroquinate dehydratase, type I (TIGR01093; EC 4.2.1.10; HMM-score: 24.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
  Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 12.1)
• TheSEED: see SAUSA300_1182
• PFAM:
  THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 214.2)
  and 5 more
  no clan defined POR; Pyruvate ferredoxin/flavodoxin oxidoreductase (PF01558; HMM-score: 136.9)
  TKC_like (CL0591) PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 40.6)
  THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 30.1)
  TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 24.4)
  TIM_barrel (CL0036) DHquinase_I; Type I 3-dehydroquinase (PF01487; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.028246
  • TAT(Tat/SPI): 0.000914
  • LIPO(Sec/SPII): 0.001441
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446727015 NCBI
• RefSeq: WP_000804328 NCBI
• UniProt: see SAUSA300_1182

⊟Protein sequence

• MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSRIKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVKPEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGDKVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQFMAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPGLSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVAPTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQSDIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQMEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQIRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPFLPHEIEEKGKIIATEIKEMV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications