PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS06675 [old locus tag: SAUSA300_1231 ]
• pan locus tag^?: SAUPAN003706000
• symbol: SAUSA300_RS06675
• pan gene symbol^?: —
• synonym:
• product: gamma-aminobutyrate permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS06675 [old locus tag: SAUSA300_1231 ]
• symbol: SAUSA300_RS06675
• product: gamma-aminobutyrate permease
• replicon: chromosome
• strand: -
• coordinates: 1348339..1349793
• length: 1455
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1348339..1349793) NCBI
• BioCyc: SAUSA300_RS06675 BioCyc
• MicrobesOnline: see SAUSA300_1231

⊟Phenotype

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⊟DNA sequence

• 1
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  1441
  
  ATGGAAGATAATAAAATGAACCGTAGTCTTAACTCAAGACACATTTCCATGATTGCTATA
  GGTGGTGCAATTGGGACTGGTCTATTTGTAGCTACTGGTAATATCATTTCTCAAGCTGGT
  CCTGGAGGCGCTATACTCGCTTATCTTGTTATTGGTGTCATGCTATATTTCTTAATGTCA
  TCAATTGGAGAGTTGGCAACATTTTATCCAGTATCAGGTTCATTCAGCTCTTATTCAACA
  CGCTTTATTGACTCATCTCTTGGCTTTACCATGGGATGGTTGTATTGGGCATTGTGGTCA
  TTAGTTACAAGTGTTGATGTCATAGTAGCGTCAAATGTGCTTTATTTCTGGGACACATTT
  AAATTTTTCCACCCCATTACTTGGAGCTTAATCTTTATTACAATTTTACTATTATTAAAC
  ATTTTTTCTGTAAAATCATTTGGAGAAACTGAGTTTTGGTTATCATTGATTAAAGTGTTA
  ACAATTATCGTATTCGTTATTTTTGGCTTTTTAATGATTTTCGGTATCTTAGGTGGTCAT
  ACATATGGATTTGAAAACTATACAAAAGGCCAAGCACCGTTTGTTGGTGGTATCTCTGGT
  TTCTTAGGCGTATTATTAGTCGCCGGATTTTCGGTTGGTGGTACAGAAGTAGTAGCAGTA
  ACTGCTGGTGAATCAGATGACCCTAAAAAGTCTATGCCTAAGGCAATTAAACAAGTATTT
  TGGCGTATTCTTTTATTCTATGTCTTATCAATTGCAGTAATTGGTGCAATTATTCCGTAC
  ACAGATCCATCATTATTAAGAGCAAGTAGTTCAATAAGTCAAAGCCCATTTACAATTGTA
  TTCGATAGAGTAGGCATAGCCTTTGCAGCATCAGTAATCAACGCGGTTATTTTAACTTCA
  TTATTATCCGCTGCAAATTCAGGTGTTTATACAACAGGCAGAATGTTGTATTCCTTAAGT
  TCAGACAAAAAAGCACCCCAATTTTTAAGTAAATTAAACAAGACAACTAAGTTACCTTTA
  AGAGCATTATTAACTACTTATGCAGTCGTTGTTATTGTTATTATTTATGCAAACTTTAAT
  TCAAATGCCGTTTTTAATTTACTTGAAATTATTGGTTCAATGATTATAGTTGTTTGGGGA
  TCAAGCATTTGGTCACAAATACGATTGCGACAAGCTATTAAAAAACAAGGTCAAGACCCT
  AATAAGGTCCTACCATATAAAGCACCTTTTTATCCATTAGGACCAATCATTGTCATCACT
  ACACTATTATTCTTGCTATTTGGTGGCTCAGTTGAATATATTTTAAAAGATCAATGGTTA
  AATGCTTTTAAAAACTTTTTACCTTTAATCATTCTAGCGTTGATTTACTTTATTCATAAA
  ATCATTCACAAAACAAAATTTGTAAAGCTAGAAACAATTAATTTAAAACCACACGATTAT
  GACAATCAAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS06675 [old locus tag: SAUSA300_1231 ]
• symbol: SAUSA300_RS06675
• description: gamma-aminobutyrate permease
• length: 484
• theoretical pI: 10.0022
• theoretical MW: 53412.7
• GRAVY: 0.676653

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 396.2)
  and 7 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 310.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 106.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 77.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 62.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 54.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 51.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 51.5)
• TheSEED: see SAUSA300_1231
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 409.2)
  and 1 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 154.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.054505
  • TAT(Tat/SPI): 0.032321
  • LIPO(Sec/SPII): 0.053984
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 446312101 NCBI
• RefSeq: WP_000389956 NCBI
• UniProt: see SAUSA300_1231

⊟Protein sequence

• MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGAILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLVTSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVLTIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVVAVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSPFTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNKTTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQAIKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLIILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_1231

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications