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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS06840 [old locus tag: SAUSA300_1259 ]
- pan locus tag?: SAUPAN003757000
- symbol: SAUSA300_RS06840
- pan gene symbol?: umuC
- synonym:
- product: DNA repair protein MucB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS06840 [old locus tag: SAUSA300_1259 ]
- symbol: SAUSA300_RS06840
- product: DNA repair protein MucB
- replicon: chromosome
- strand: +
- coordinates: 1386229..1387491
- length: 1263
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1386229..1387491) NCBI
- BioCyc: SAUSA300_RS06840 BioCyc
- MicrobesOnline: see SAUSA300_1259
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261GTGTATAATTATCATTTATTAGAAGATAGGGATGTTCTATGTATTGACCAAAAAAGTTTT
TTTGCGAGTGTTTCTTGTATTGAAAAGGGGCTAGATCCATTAGAAACAAAGCTAGCTGTT
GTTGCAGATACTAAGCGTCAGGGTTCTGTAATATTGGCTGCGACACCTAAATTAAAAGAA
TTAGGCATCAAGACAGGGTCGCGATTGTTTGAAATACCACATAGAAATGATATTTACATT
ATCAATCCAAGTATGCGTAAATATCTTAATGTTTCAGTTGCTATTTCTAAGATTGCATTG
CGTTATATTCCACCTGAAGATTTACACCAATATAGTATTGACGAATTTTTTATGGATGTT
ACTGATAGCTATCATAGATTTAGTTCTACAGTACATGCATTTTGCGAAAGACTTAAACGT
GAAATTTATGAAGAAACAGGCATTTATTGTACTGTGGGCATTGGTTCTAATATGTTATTA
AGTAAAATTGCTATGGATGTTGAAGCGAAGCATAGTCAAAATGGTATAGCTGAATGGCGA
TATCAAGATGTACCAACGAAATTATGGCCAATTCAGCCCTTGCGAGATTTTTGGGGTATT
AATCGTCGAACAGAAGCCAAATTGAATAAAAGAGGAATTTTTACTATAGGAGATTTAGCG
AAATATCCATATAAATTTTTAAAAAAAGAGTTCGGTATTTTAGGTGTTGATATGCATCTA
CATGCGAATGGGATAGATCAGAGTAAAGTACGTGAAAAGCACAAGATCAGCAATCCATCG
ATATGCAAAAGTCAAATATTAATGAGAGATTATCATTTTGATGAAGCAAAAGTAGTAATG
CAAGAGTTAATTGAAGATGTTGCTAGCAGAGTTCGAGCAAGAAAAAAAGTGGCAAGAACG
ATACATTTTGCCTTTGGCTATAGTGATGAAGGCGGTGTACATAAGCAATATACTTTGAAA
GATCCAACAAACTTAGAAAAAGATATTTATAAAGTAGTAATGCATTTCGCAGATAAATTA
TGTAATAAACAAGCACTATATCGTACGCTAAGTATATCTTTGAGTCAATTTATTAATGAG
GATGAGCGACAGTTAAGTCTGTTTGAAGATGAATACCAACGCAAACGTGACGAATGTCTA
GCTAAAACGATAGACCAATTACATTTGAAATACGGCAAAGGTATTGTGTCCAAAGCAGTA
TCGTTTACAGAAGCAGGTACAAAACACGGCAGATTAGGTTTAATGGCTGGACATAAAATG
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS06840 [old locus tag: SAUSA300_1259 ]
- symbol: SAUSA300_RS06840
- description: DNA repair protein MucB
- length: 420
- theoretical pI: 9.23261
- theoretical MW: 48426.6
- GRAVY: -0.393571
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SAUSA300_1259
- PFAM: no clan defined IMS; impB/mucB/samB family (PF00817; HMM-score: 117.2)and 2 moreIMS_C; impB/mucB/samB family C-terminal domain (PF11799; HMM-score: 56.2)HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 37.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004875
- TAT(Tat/SPI): 0.002363
- LIPO(Sec/SPII): 0.013254
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446205161 NCBI
- RefSeq: WP_000283016 NCBI
- UniProt: see SAUSA300_1259
⊟Protein sequence[edit | edit source]
- MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADTKRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRYIPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLLSKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGDLAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEAKVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVMHFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYGKGIVSKAVSFTEAGTKHGRLGLMAGHKM
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: LexA see SAUSA300_1259
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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