PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS07015 [old locus tag: SAUSA300_1291 ]
• pan locus tag^?: SAUPAN003811000
• symbol: SAUSA300_RS07015
• pan gene symbol^?: dapI
• synonym:
• product: hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS07015 [old locus tag: SAUSA300_1291 ]
• symbol: SAUSA300_RS07015
• product: hydrolase
• replicon: chromosome
• strand: +
• coordinates: 1421559..1422710
• length: 1152
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1421559..1422710) NCBI
• BioCyc: SAUSA300_RS07015 BioCyc
• MicrobesOnline: see SAUSA300_1291

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATGAATTAGAATTTGTTACGAAACATCGCCGTCATTTACATCAACATCCTGAATTA
  AGCTTACATGAATTTGAAACAACTGCTTATATTAAAGCGTTTTTAGATAGTTTAAATATT
  AAATACGATTGCCCATTGGAAACTGGCGTCATTGCATACTTAGAAGGTAATGGCTCACAT
  ACGATAGCGTATAGAGCTGATATTGATGCGTTACCTATTTTAGAGGAAAATGATGTGCCT
  TATCGCAGTCAATCTGATCATGTGATGCATGCTTGTGGACATGATGGTCATACAACTGCA
  TTAATGCTTTTTGTACAACGTTGCAAAGACATGCAAGATGCAGGTCAATTACCGCAAAAT
  GTCGTTTTCATTTTCCAACCTGCAGAAGAAACTGGTGGCGGTGCAAATCGATTAATAAAA
  GCCGGTGCCTTTGATAAGTATCCAATTGAAGCGGTATTTGGTATTCATGTTAACCCATTT
  GCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTAT
  CGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGT
  GGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAAC
  GGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACT
  GTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACA
  ATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAT
  TGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAAATTACGTACT
  CAAATAGAGGACGCCTTAATAAAAGCTGATTTAAATGTCTATGACAAACCAACGCCATTC
  TTATTTGGGGAAGATTTTAGTTTTTATGGTCAACAACTAGCTCCAGCTTACTTTGTTTTT
  ATAGGAACACGAAATGAAGATAAAGGTTTTGTAACTGGTTTGCACACATCACATTTAAAT
  TTTGATGAAAAAGTGTTAATAAACGTGGTTAATTTTTACGAAAATTTATTAAATAATTAC
  AAAGAGGTGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS07015 [old locus tag: SAUSA300_1291 ]
• symbol: SAUSA300_RS07015
• description: hydrolase
• length: 383
• theoretical pI: 5.35281
• theoretical MW: 43143.3
• GRAVY: -0.252219

⊟Function

• reaction:
  EC 3.5.1.14?  ExPASy
  N-acyl-aliphatic-L-amino acid amidohydrolase An N-acyl-aliphatic-L-amino acid + H₂O = an aliphatic L-amino acid + a carboxylate An N-acetyl-L-cysteine-S-conjugate + H₂O = an L-cysteine-S-conjugate + acetate
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 279.6)
  and 2 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 20.7)
  Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 20.4)
• TheSEED: see SAUSA300_1291
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 117.3)
  and 2 more
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 22.7)
  DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002731
  • TAT(Tat/SPI): 0.000328
  • LIPO(Sec/SPII): 0.000364
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446926537 NCBI
• RefSeq: WP_001003793 NCBI
• UniProt: see SAUSA300_1291

⊟Protein sequence

• MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDCPLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALMLFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPFADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFHLNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLLFNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLAPAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: L-box, CodY see SAUSA300_1291

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications