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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS07140 [old locus tag: SAUSA300_1311 ]
- pan locus tag?: SAUPAN003843000
- symbol: SAUSA300_RS07140
- pan gene symbol?: murG
- synonym:
- product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS07140 [old locus tag: SAUSA300_1311 ]
- symbol: SAUSA300_RS07140
- product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
- replicon: chromosome
- strand: -
- coordinates: 1444430..1445500
- length: 1071
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1444430..1445500) NCBI
- BioCyc: SAUSA300_RS07140 BioCyc
- MicrobesOnline: see SAUSA300_1311
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGACGAAAATCGCATTTACCGGAGGGGGAACAGTTGGACACGTATCAGTAAATTTAAGT
TTAATTCCAACTGCATTATCACAAGGTTATGAAGCGCTTTATATTGGTTCTAAAAATGGT
ATTGAAAGAGAAATGATTGAATCACAACTACCAGAAATTAAGTATTATCCTATTTCGAGT
GGTAAATTAAGAAGATATATTTCTTTAGAAAATGCCAAAGACGTATTTAAAGTATTGAAA
GGTATTCTTGATGCTCGTAAAGTTTTGAAAAAAGAAAAACCTGATCTATTATTTTCAAAA
GGTGGATTTGTATCTGTGCCTGTTGTTATTGCAGCCAAATCATTAAATATACCAACTCTT
ATTCATGAATCTGACTTAACACCAGGATTAGCGAATAAGATAGCACTTAAATTTGCCAAG
AAAATATATACAACATTTGAAGAAACGCTAAACTACTTACCTAAAGAGAAAGCTGATTTT
ATTGGAGCAACAATTCGAGAAGATTTAAAAAATGGTAATGCACATAATGGTTATCAATTA
ACAGGCTTTAATGAAAATAAAAAAGTTTTACTCGTTATGGGTGGAAGCTTAGGAAGTAAA
AAATTAAATAGCATTATTCGCGAAAACTTAGATGCATTATTACAACAATATCAAGTGATA
CATTTAACTGGTAAAGGATTAAAAGATGCTCAAGTTAAAAAATCAGGATATATACAATAT
GAATTTGTTAAAGAGGATTTAACAGATTTATTAGCAATTACGGATACAGTAATAAGTAGA
GCTGGATCAAATGCGATTTATGAGTTCTTAACATTACGTATACCAATGTTATTAGTACCA
TTAGGTTTAGATCAATCCCGAGGCGACCAAATTGACAATGCAAATCATTTTGCTGATAAA
GGATATGCTAAAGCGATTGATGAAGAACAATTAACAGCACAAATTTTATTACAAGAACTA
AATGAAATGGAACAGGAAAGAACTCGAATTATCAATAATATGAAATCGTATGAACAAAGT
TATACGAAAGAAGCTTTATTTGATAAGATGATTAAAGACGCATTGAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS07140 [old locus tag: SAUSA300_1311 ]
- symbol: SAUSA300_RS07140
- description: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
- length: 356
- theoretical pI: 9.13983
- theoretical MW: 39696.5
- GRAVY: -0.229494
⊟Function[edit | edit source]
- reaction: EC 2.4.1.227? ExPASyUndecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 244)and 2 moreCellular processes Toxin production and resistance glycosyltransferase, MGT family (TIGR01426; EC 2.4.1.-; HMM-score: 40.6)Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 29.7)
- TheSEED: see SAUSA300_1311
- PFAM: GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 128.5)and 6 moreGlyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 95)UDPGT; UDP-glucoronosyl and UDP-glucosyl transferase (PF00201; HMM-score: 29.7)Glyco_trans_1_3; Glycosyl transferase family 1 (PF13528; HMM-score: 17.9)no clan defined Oberon_cc; Coiled-coil region of Oberon (PF16312; HMM-score: 14.2)GT-B (CL0113) Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 13.6)no clan defined TFIIA_gamma_N; Transcription initiation factor IIA, gamma subunit, helical domain (PF02268; HMM-score: 13.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.310983
- TAT(Tat/SPI): 0.006498
- LIPO(Sec/SPII): 0.013024
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446083058 NCBI
- RefSeq: WP_000160913 NCBI
- UniProt: see SAUSA300_1311
⊟Protein sequence[edit | edit source]
- MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIEREMIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGGFVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNMKSYEQSYTKEALFDKMIKDALN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS01250 L-lactate dehydrogenase [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02845 elongation factor G [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS03415 dihydroxyacetone kinase subunit L [1] (data from MRSA252) SAUSA300_RS03530 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS04905 enoyl-ACP reductase [1] (data from MRSA252) SAUSA300_RS08910 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS09040 universal stress protein UspA [1] (data from MRSA252) SAUSA300_RS11315 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252) SAUSA300_RS11380 uracil phosphoribosyltransferase [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS13795 hydroxymethylglutaryl-CoA synthase [1] (data from MRSA252) SAUSA300_RS14295 arginine deiminase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)