PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS07345 [old locus tag: SAUSA300_1347 ]
• pan locus tag^?: SAUPAN003916000
• symbol: SAUSA300_RS07345
• pan gene symbol^?: birA
• synonym:
• product: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS07345 [old locus tag: SAUSA300_1347 ]
• symbol: SAUSA300_RS07345
• product: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor
• replicon: chromosome
• strand: -
• coordinates: 1514019..1514990
• length: 972
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1514019..1514990) NCBI
• BioCyc: SAUSA300_RS07345 BioCyc
• MicrobesOnline: see SAUSA300_1347

⊟Phenotype

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⊟DNA sequence

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  ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
  TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAAGTGATT
  GACCAATTAAAGTTAGAGGGATGTAAAATAGATTCAGTAAATCATAAAGGGCATTTATTA
  CAACAGCTCCCAGATATTTGGTATCAAGGTATAATAGACCAATATACAAAAAGTTCTGCT
  TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
  TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
  GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
  TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
  ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
  ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
  GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
  GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
  AGATATCAATTTTTAGAGATATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
  ACGTTACCTTTTTCTGAAATTCGTGAAGAATATACTGCAGCTTCTAATATTTGGAATAGA
  ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
  GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
  ATAGATTTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS07345 [old locus tag: SAUSA300_1347 ]
• symbol: SAUSA300_RS07345
• description: bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor
• length: 323
• theoretical pI: 5.92816
• theoretical MW: 37086.7
• GRAVY: -0.360681

⊟Function

• reaction:
  EC 6.3.4.15?  ExPASy
  Biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair biotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 180.1)
  and 4 more
  Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 32.2)
  Signal transduction Regulatory functions DNA interactions TyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 12.5)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.7)
• TheSEED: see SAUSA300_1347
• PFAM:
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 53.4)
  tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 48.7)
  and 13 more
  BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.8)
  TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 19.7)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.3)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 17.1)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.8)
  MarR; MarR family (PF01047; HMM-score: 14.8)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.7)
  RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.6)
  Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 13.2)
  no clan defined PulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)
  HTH (CL0123) HTH_46; Winged helix-turn-helix DNA binding (PF15977; HMM-score: 12.3)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by BirA, TF important in Biotin biosynthesis: see SAUSA300_1347

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010951
  • TAT(Tat/SPI): 0.000494
  • LIPO(Sec/SPII): 0.001853
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445972064 NCBI
• RefSeq: WP_000049919 NCBI
• UniProt: see SAUSA300_1347

⊟Protein sequence

• MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications