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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS07540 [old locus tag: SAUSA300_1381 ]
- pan locus tag?:
- symbol: SAUSA300_RS07540
- pan gene symbol?: —
- synonym:
- product: leucotoxin LukD
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS07540 [old locus tag: SAUSA300_1381 ]
- symbol: SAUSA300_RS07540
- product: leucotoxin LukD
- replicon: chromosome
- strand: -
- coordinates: 1546170..1547153
- length: 984
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1546170..1547153) NCBI
- BioCyc: SAUSA300_RS07540 BioCyc
- MicrobesOnline: see SAUSA300_1381
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATTGATATGAAAAAAATAGTCAAATCATCAGTTGTTACATCAATTGCATTGCTTTTGCTA
TCCAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGTGAGAAAAAGGTTGATGAT
AAAATTACTTTGTACAAAACAACTGCAACATCAGATTCCGATAAGTTAAAAATTTCTCAG
ATTTTAACTTTTAATTTTATTAAAGATAAAAGTTATGATAAAGATACATTAATACTCAAA
GCTGCTGGAAACATTTATTCTGGCTATACAAAGCCAAATCCAAAAGACACTATTAGTTCT
CAATTTTATTGGGGTTCTAAGTACAACATTTCAATTAATTCAGATTCTAATGACTCAGTA
AACGTTGTAGATTATGCACCTAAAAATCAAAATGAAGAATTTCAAGTACAACAAACGGTA
GGTTATTCTTATGGTGGAGATATTAATATCTCTAACGGCTTATCAGGTGGAGGTAATGGT
TCAAAATCTTTTTCAGAGACAATTAACTATAAACAAGAAAGCTATAGAACTAGCTTAGAT
AAAAGAACTAATTTCAAAAAAATTGGTTGGGATGTTGAAGCACATAAAATTATGAATAAT
GGTTGGGGACCATATGGCAGAGATAGTTATCATTCAACTTATGGTAATGAAATGTTTTTA
GGCTCAAGACAAAGCAACTTAAATGCTGGACAAAACTTCTTGGAATATCACAAAATGCCA
GTGTTATCCAGAGGTAACTTCAATCCAGAATTTATTGGTGTCCTATCTCGAAAACAAAAC
GCTGCAAAAAAATCGAAAATTACTGTTACTTATCAAAGAGAAATGGATAGATATACAAAC
TTTTGGAATCAACTTCACTGGATAGGTAATAATTATAAAGATGAAAATAGAGCAACTCAT
ACATCAATTTATGAAGTTGATTGGGAAAATCATACAGTTAAATTAATAGATACTCAATCT
AAGGAAAAAAATCCTATGAGCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS07540 [old locus tag: SAUSA300_1381 ]
- symbol: SAUSA300_RS07540
- description: leucotoxin LukD
- length: 327
- theoretical pI: 9.47779
- theoretical MW: 37208.2
- GRAVY: -0.790826
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 456)
- TheSEED: see SAUSA300_1381
- PFAM: Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 272)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
- LocateP:
- SignalP: Signal peptide SP(Sec/SPI) length 26 aa
- SP(Sec/SPI): 0.992707
- TAT(Tat/SPI): 0.000766
- LIPO(Sec/SPII): 0.003764
- Cleavage Site: CS pos: 26-27. VDA-AQ. Pr: 0.9573
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 640499474 NCBI
- RefSeq: WP_024937002 NCBI
- UniProt: see SAUSA300_1381
⊟Protein sequence[edit | edit source]
- MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQFYWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNGSKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEMFLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMDRYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SaeR see SAUSA300_1381
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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