From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08315 [old locus tag: SAUSA300_1524 ]
  • pan locus tag?: SAUPAN004145000
  • symbol: SAUSA300_RS08315
  • pan gene symbol?: ccpN
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08315 [old locus tag: SAUSA300_1524 ]
  • symbol: SAUSA300_RS08315
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1675315..1675938
  • length: 624
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATAGAACTCAGTCAAAGACAAGAACGAATCATCGAAATTGTTAAAACTAAAGGACCCATT
    ACTGGTGAACAAATAGCAGATAAGTTGAATTTAACAAGAGCAACGCTTAGACCAGATTTA
    GCGATATTAACAATGTCAGGTTTTTTAGAAGCGCGTCCCCGAGTTGGATATTATTATTCA
    GGTAAATCAAAAGGCAAATTTTTTAATGAAAAACTTCGACAATTTGAGGTTAAGGACTAT
    ATGTCTCAACCTGTTGTGCTAAGAGAGAATACAACAGTTTATGACGCTATTTGTACAATT
    TTTTTAGAAGATGTAAGTACATTATTTATTATTAATGAAGATAACGATTTTGTTGGTGTG
    TGTTCAAGAAAAGATTTATTAAGAGCTTCAATGATTGGAGCAGATATTCATAAAGTACCT
    ATCAGTGTAAATATGACACGTATGCCTAATGTCACTTATTTAGAGGAAAGCGAATTAGTC
    ATATACGCAGCAGATAGAATGATTGAAAAAGAAATTGATTCGATTCCAATTGTAAGAAAA
    AAAGATAATCAAAAGTATGAAGTAATTGGAAGAATTTCCAAAACAACAATAGCTAAGTTA
    TTAGTAGCATTATATAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    624

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08315 [old locus tag: SAUSA300_1524 ]
  • symbol: SAUSA300_RS08315
  • description: hypothetical protein
  • length: 207
  • theoretical pI: 8.60375
  • theoretical MW: 23735.5
  • GRAVY: -0.195169

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 28.4)
    and 3 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 18.7)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 17.5)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 14)
  • TheSEED: see SAUSA300_1524
  • PFAM:
    no clan defined CBS; CBS domain (PF00571; HMM-score: 54.5)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 49.5)
    and 10 more
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 25.6)
    MarR; MarR family (PF01047; HMM-score: 20.4)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 16.5)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 15.4)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 14.2)
    TFIIE_alpha; TFIIE alpha subunit (PF02002; HMM-score: 13.7)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 13.6)
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 13.1)
    DUF977; Bacterial protein of unknown function (DUF977) (PF06163; HMM-score: 12.8)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006653
    • TAT(Tat/SPI): 0.00173
    • LIPO(Sec/SPII): 0.001151
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILTMSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFLEDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELVIYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS08315&oldid=73272"