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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08935 [old locus tag: SAUSA300_1638 ]
  • pan locus tag?: SAUPAN004316000
  • symbol: SAUSA300_RS08935
  • pan gene symbol?: phoR
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08935 [old locus tag: SAUSA300_1638 ]
  • symbol: SAUSA300_RS08935
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1795536..1797197
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTTGTT
    ACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAACAA
    GCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGATAAA
    GTTAAAAAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAGCATGATAAAAAG
    GTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAAAGG
    GACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTACAAT
    AACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATTTTGACATGTTTAGTT
    GTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCCATT
    CAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTACCT
    GAAAGTAATGTTGTAGAAACTAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGACGC
    CTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATTGAA
    AATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAATAAA
    GCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGCTAT
    GTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATTTAT
    GAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGCGTT
    CCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGATATA
    ACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACATGAA
    TTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCAAAA
    AATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGAATA
    GAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTAGAT
    ACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACTCAA
    GCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAAGCA
    CAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTATTCT
    TATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTCGAA
    GTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGATTT
    TATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCAATT
    ACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGCAAA
    GGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08935 [old locus tag: SAUSA300_1638 ]
  • symbol: SAUSA300_RS08935
  • description: sensor histidine kinase
  • length: 553
  • theoretical pI: 8.11061
  • theoretical MW: 63772.9
  • GRAVY: -0.20217

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
    and 10 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 141.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 27.8)
    Metabolism Energy metabolism Photosynthesis transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  • TheSEED: see SAUSA300_1638
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 91)
    and 10 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 70.5)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.1)
    PAS_4; PAS fold (PF08448; HMM-score: 25)
    PAS_7; PAS fold (PF12860; HMM-score: 22.5)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 20.4)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 18.6)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 18.4)
    PAS_Fold (CL0183) PAS_8; PAS domain (PF13188; HMM-score: 17.7)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 14.1)
    MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.595768
    • TAT(Tat/SPI): 0.004057
    • LIPO(Sec/SPII): 0.009455
    • Cleavage Site: CS pos: 28-29. IHA-SI. Pr: 0.3568
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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