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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10735 [old locus tag: SAUSA300_1957 ]
  • pan locus tag?: SAUPAN001394000
  • symbol: SAUSA300_RS10735
  • pan gene symbol?: dnaD2
  • synonym:
  • product: replication protein DnaD

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10735 [old locus tag: SAUSA300_1957 ]
  • symbol: SAUSA300_RS10735
  • product: replication protein DnaD
  • replicon: chromosome
  • strand: -
  • coordinates: 2114638..2115531
  • length: 894
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACTGGTTGGATAAGTATTGATCGCTCAATTCAAAATCATTGGCTATTTAAAGAAAAG
    AGAACATTTTCAAAGTTTGAAGCATGGATATATTTACTCATGGAAGCGAATCATTCAAAG
    GCAAAAGTGCCTATAGGAAACCAAATTGTAACCGTTGAAAGAGGACAAAGATTAACATCT
    ATTTTGACCTTGTCTGACCTTTTTAACTGGTCACGATTTAAAGTGAAAACCTTCCTTGAC
    TTACTCGAGAGTGATGGAATGTTAGAAGTCAAAACAACATCAAAATATACCCTTATAACC
    ATTGTCAATTATGACTTTTATCAAAGTGAGCAGGGCAGGAACCAACATCAAAACGACATC
    AAACCAACATCAAAACAACATCAGTCAAACATCAACCCAACATCAAAACAACATCAAACC
    AACACAAACAATAATGATAATAAAGATAATAATGAAAAGAATGTGAATAATGAGAAGAAG
    AAGACAACCGCCTTCGACTTCTTCCAAGATAACGGATTCGGTTTCATAACTTCTTACAAT
    TTAGACGATTTAAATTATTATCTTGATTCATTTGAAAATGATTCAGATGAAATAGTTACC
    GCATCACTTAAAATCGCTAAAGACAGAAACAAAGTTACTTGGGGATATGCTAAAAGCATT
    TTGAATACATGGCTTAATGCAAACTTGAAATCTATTGAACAAGTACGTGCATTTGAAAAG
    CAACAACTTGAAAGCAAAAAACAAAATTATAAACCTTTCGTTAAACAATCAAAAGAAAAA
    ACACCCAAATGGCTCACAGACAGCACGAGAGAAACGAAAACGCCGGAAGTAGATGAAAAC
    CTTGAGAAAGACAGAGAAGCTTTTATTAAGCGTCTAAATAGCAAATGGGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    894

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS10735 [old locus tag: SAUSA300_1957 ]
  • symbol: SAUSA300_RS10735
  • description: replication protein DnaD
  • length: 297
  • theoretical pI: 9.58803
  • theoretical MW: 34981.9
  • GRAVY: -0.967677

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 79.1)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 79.1)
  • TheSEED: see SAUSA300_1957
  • PFAM:
    no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 47.6)
    and 3 more
    HTH (CL0123) Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 14)
    no clan defined UPF0561; Uncharacterised protein family UPF0561 (PF10573; HMM-score: 11.8)
    CPSF100_C; Cleavage and polyadenylation factor 2 C-terminal (PF13299; HMM-score: 10.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007995
    • TAT(Tat/SPI): 0.000465
    • LIPO(Sec/SPII): 0.000664
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVPIGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIVNYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKKKTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAKSILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDENLEKDREAFIKRLNSKWE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]