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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
  • pan locus tag?: SAUPAN005132000
  • symbol: SAUSA300_RS10815
  • pan gene symbol?: —
  • synonym:
  • product: ATP-dependent helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
  • symbol: SAUSA300_RS10815
  • product: ATP-dependent helicase
  • replicon: chromosome
  • strand: +
  • coordinates: 2123965..2124897
  • length: 933
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGC
    CCTTGCGAAGTTGCTGTATCTTTAATTAAGGATTTATCAATAGTAAAAGTTTATTCATCT
    TATATTAATCCTCCTAATAATAGATATAACTTGAAAAACGCTAAAATACATAAAATACCT
    GAAGATGTCATATTAAAAGCACCTAAATATCCAGATATTTACCAAGAAATTCTCTATCTT
    TTAAAAGAATCACATTTAATTATTGCTCATAATGCACTTTTTGATATTTCAGTATTAAAA
    AATACTAATAATTATTATGACTTACCTGTTCCAAACTTCATGTATGTCGATAGTATAAAT
    ATCTTTAGAAGCTTCCACGCAATCTCTAGTTTTAAATTAGAAAATTTGTGTAGCTTATAT
    GATATCGATAAAGAAAAATTACATTCTGCTAAATTTGACGTGCTAGCTTTATCGAAGATG
    TTGATATCACTCGCTAAAAACAATCAGCATTATAGTGTATTAAAATTAATACATTATATG
    CCTAAGCAATACATTAGATTTAGCAAATATTCTAACTCTCCAACTAAACTTTTCGATTCA
    GGATTTCAAAAAATTCATATGAAAATATCTGAGATTAATAAAATAGAAGTGGAAAGTGTA
    ATCCCTATTTTAAAAGATAAAAATGTTGTTTTTACAGGTAATTTTGACACTGAAAAACAA
    GATTTAATGATATTAACTAGAAAGAAAGGAGCTTATATCAGAAGTGACGTAACTGCAAAA
    ACAGATATTTTAGTCGAAGGTGTTCAAGATGATAAATATAAAGATGTGAACGGACTAGTT
    TCAAAACAACGAAAAGCTCGAGAATATGTTGGAAATGGTGCAAAAATTCAATTTTTAAAT
    GAAGAAGACTTAATAAATTTAATAAAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
  • symbol: SAUSA300_RS10815
  • description: ATP-dependent helicase
  • length: 310
  • theoretical pI: 9.1498
  • theoretical MW: 35900.4
  • GRAVY: -0.280645

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 62.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 62.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 54.2)
    and 2 more
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 42.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA ligase, NAD-dependent (TIGR00575; EC 6.5.1.2; HMM-score: 22.9)
  • TheSEED: see SAUSA300_1970
  • PFAM:
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 45.6)
    and 3 more
    BRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 16.4)
    RNase_H (CL0219) DUF5051; 3' exoribonuclease, RNase T-like (PF16473; HMM-score: 15.4)
    DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008508
    • TAT(Tat/SPI): 0.000238
    • LIPO(Sec/SPII): 0.000951
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINPPNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNTNNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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