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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
- pan locus tag?: SAUPAN005132000
- symbol: SAUSA300_RS10815
- pan gene symbol?: —
- synonym:
- product: ATP-dependent helicase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
- symbol: SAUSA300_RS10815
- product: ATP-dependent helicase
- replicon: chromosome
- strand: +
- coordinates: 2123965..2124897
- length: 933
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2123965..2124897) NCBI
- BioCyc: SAUSA300_RS10815 BioCyc
- MicrobesOnline: see SAUSA300_1970
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGC
CCTTGCGAAGTTGCTGTATCTTTAATTAAGGATTTATCAATAGTAAAAGTTTATTCATCT
TATATTAATCCTCCTAATAATAGATATAACTTGAAAAACGCTAAAATACATAAAATACCT
GAAGATGTCATATTAAAAGCACCTAAATATCCAGATATTTACCAAGAAATTCTCTATCTT
TTAAAAGAATCACATTTAATTATTGCTCATAATGCACTTTTTGATATTTCAGTATTAAAA
AATACTAATAATTATTATGACTTACCTGTTCCAAACTTCATGTATGTCGATAGTATAAAT
ATCTTTAGAAGCTTCCACGCAATCTCTAGTTTTAAATTAGAAAATTTGTGTAGCTTATAT
GATATCGATAAAGAAAAATTACATTCTGCTAAATTTGACGTGCTAGCTTTATCGAAGATG
TTGATATCACTCGCTAAAAACAATCAGCATTATAGTGTATTAAAATTAATACATTATATG
CCTAAGCAATACATTAGATTTAGCAAATATTCTAACTCTCCAACTAAACTTTTCGATTCA
GGATTTCAAAAAATTCATATGAAAATATCTGAGATTAATAAAATAGAAGTGGAAAGTGTA
ATCCCTATTTTAAAAGATAAAAATGTTGTTTTTACAGGTAATTTTGACACTGAAAAACAA
GATTTAATGATATTAACTAGAAAGAAAGGAGCTTATATCAGAAGTGACGTAACTGCAAAA
ACAGATATTTTAGTCGAAGGTGTTCAAGATGATAAATATAAAGATGTGAACGGACTAGTT
TCAAAACAACGAAAAGCTCGAGAATATGTTGGAAATGGTGCAAAAATTCAATTTTTAAAT
GAAGAAGACTTAATAAATTTAATAAAGGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS10815 [old locus tag: SAUSA300_1970 ]
- symbol: SAUSA300_RS10815
- description: ATP-dependent helicase
- length: 310
- theoretical pI: 9.1498
- theoretical MW: 35900.4
- GRAVY: -0.280645
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 62.5)DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 62.1)DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 54.2)and 2 moreDNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 42.4)DNA metabolism DNA replication, recombination, and repair DNA ligase, NAD-dependent (TIGR00575; EC 6.5.1.2; HMM-score: 22.9)
- TheSEED: see SAUSA300_1970
- PFAM: RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 45.6)and 3 moreBRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 16.4)RNase_H (CL0219) DUF5051; 3' exoribonuclease, RNase T-like (PF16473; HMM-score: 15.4)DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008508
- TAT(Tat/SPI): 0.000238
- LIPO(Sec/SPII): 0.000951
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446682336 NCBI
- RefSeq: WP_000759682 NCBI
- UniProt: see SAUSA300_1970
⊟Protein sequence[edit | edit source]
- MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINPPNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNTNNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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