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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10865 [old locus tag: SAUSA300_1978 ]
  • pan locus tag?: SAUPAN005203000
  • symbol: SAUSA300_RS10865
  • pan gene symbol?: fhuD1
  • synonym:
  • product: ferrichrome-binding protein FhuD

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10865 [old locus tag: SAUSA300_1978 ]
  • symbol: SAUSA300_RS10865
  • product: ferrichrome-binding protein FhuD
  • replicon: chromosome
  • strand: -
  • coordinates: 2133798..2134709
  • length: 912
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAGATTAATTGGAATTTTATTATGTAATTTATTTATTTTGACTGCGTGTTCAGCA
    TCTGTCGATAAAACAAGTAACTCGACAAAGACTACAGACTATAAAATTGAAAATGGTGAA
    ACACTGAAAGTACCAGAGAAACCTAAAAGAGTTGCTGTATTAACTGGATTTTATGTTGGT
    GATTTTATAAAGTTAGGAATCAAACCAATTGCTGTTTCAGATATAACTAAAGATTCTTCA
    ATTTTAAAACCTTATTTAAAAGGGGTTGATTATATTGGAGAAAATGATGTTGAAAGAGTT
    GCTAAAGCAAAACCAGATTTAATTGTTGTAGATGCTATGGATAAAAATATAAAAAAATAC
    CAAAAAATAGCGCCAACAATCCCATATACATACAATAAATACAATCATAAAGAAATATTA
    AAAGAAATAGGCAAGTTGACTAATAATGAAGATAAAGCTAAAAAATGGATTGAAGAGTGG
    GACGATAAAACTAGAAAAGATAAAAAAGAAATTCAAAGTAAAATTGGTCAAGCAACAGCA
    TCTGTGTTTGAACCAGATGAAAAGCAAATATACATATATAACTCTACATGGGGTCGTGGT
    TTGGATATTGTTCATGATGCATTCGGTATGCCAATGACAAAGCAATATAAAGATAAATTA
    CAAGAAGATAAAAAAGGCTATGCTTCCATTTCAAAAGAAAATATTAGTAAATATGCTGGT
    GATTATATATTTTTAAGTAAACCTTCGTACGGAAAATTTGATTTTGAAAAAACACATACA
    TGGCAGAATATTGAAGCTGTAAAAAAAGGACATGTAATTTCATATAAAGCAGAAGATTAT
    TGGTTCACAGATCCTATTACATTAGAACATTTGAGAAGTAAATTAAAAAAAGAAATTTTA
    AATAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    912

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS10865 [old locus tag: SAUSA300_1978 ]
  • symbol: SAUSA300_RS10865
  • description: ferrichrome-binding protein FhuD
  • length: 303
  • theoretical pI: 9.74449
  • theoretical MW: 34842.9
  • GRAVY: -0.646865

Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 26.4)
    and 1 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 15.6)
  • TheSEED: see SAUSA300_1978
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 174.4)
    and 2 more
    no clan defined RXLR; RXLR phytopathogen effector protein, Avirulence activity (PF16810; HMM-score: 15.1)
    NADH_dhqG_C; NADH-ubiquinone oxidoreductase subunit G, C-terminal (PF09326; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.81
    • Cellwall Score: 0.09
    • Extracellular Score: 0.1
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000952
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.998672
    • Cleavage Site: CS pos: 17-18. LTA-CS. Pr: 0.9992
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKVPEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAKAKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEWDDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDPITLEHLRSKLKKEILNKK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]