From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11110 [old locus tag: SAUSA300_2020 ]
  • pan locus tag?: SAUPAN005330000
  • symbol: SAUSA300_RS11110
  • pan gene symbol?:
  • synonym:
  • product: SprT family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11110 [old locus tag: SAUSA300_2020 ]
  • symbol: SAUSA300_RS11110
  • product: SprT family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2181964..2182419
  • length: 456
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATAATGACAAACTTCAGCGAATGGTTGAGAATCTTTCGGAAGAAAAATTTGGACGT
    ACGTTTCGACATTGTGCATATTTTAATAAAAGGTTACGTACAACAGGCGGACGTTATCTT
    TTAAAGTCCCATGATATTGAAATTAATCCAAAACAATATGAGCATTATGGAGAAGATGCT
    GTTGTAAAAATTATACTGCACGAATTGTGTCATTATCACTTGCATATTGCTGGTAAAGGT
    TATCAACATAAAGATCAAGATTTTAAACGATTGAGCCAACAGGTTGGAGCGCCTAGATTT
    TGTAACAGCATTGAAAGCTATCAACAACGGGCAAATTATGAATATTATTGCACTAAATGT
    CATGCAAAATATATACGTATCCGTAAAGTTGATACGAATCGTATGCGTTGCGGACATTGC
    AATGGAAAATTAAGAATGAAAAGACAATTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    456

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS11110 [old locus tag: SAUSA300_2020 ]
  • symbol: SAUSA300_RS11110
  • description: SprT family protein
  • length: 151
  • theoretical pI: 10.0258
  • theoretical MW: 18185.8
  • GRAVY: -1.01589

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 21.5)
  • TheSEED: see SAUSA300_2020
  • PFAM:
    Peptidase_MA (CL0126) SprT-like; SprT-like family (PF10263; HMM-score: 57)
    and 5 more
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_SprT; SprT-like zinc ribbon domain (PF17283; HMM-score: 41.6)
    Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 21.2)
    no clan defined FlhC; Flagellar transcriptional activator (FlhC) (PF05280; HMM-score: 13.7)
    Zn_Beta_Ribbon (CL0167) zinc_ribbon_12; Probable zinc-ribbon domain (PF11331; HMM-score: 10.6)
    zf-RRN7; Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 (PF11781; HMM-score: 10.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00798
    • TAT(Tat/SPI): 0.000876
    • LIPO(Sec/SPII): 0.001434
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSHDIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCNSIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]