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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11900 [old locus tag: SAUSA300_2159 ]
  • pan locus tag?: SAUPAN005606000
  • symbol: SAUSA300_RS11900
  • pan gene symbol?:
  • synonym:
  • product: aldo/keto reductase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11900 [old locus tag: SAUSA300_2159 ]
  • symbol: SAUSA300_RS11900
  • product: aldo/keto reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2336350..2337198
  • length: 849
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATCATATTGAAATAAGTAAAGATGTAAAGATACCAGTATTAGGTTTCGGAGTATTT
    CAAATTCCTCAAGAACAAACAGCAGAAGCGGTTAAAGAAGCAATTAAAGCGGGTTATAGA
    CATATTGATACAGCACAAAGTTATTTAAATGAAACTGAAGTAGGTCAAGGTATTGAAGCT
    TCAGGTATTGATAGAAGTGAACTATTCATTACGACTAAAGTATGGATTGAAAATGTTAAC
    TATGAAGATACTATCAAATCAATCGAACGATCATTACAACGATTAAATTTGGACTATCTT
    GATCTAGTGCTTATTCACCAACCTTACAATGACGTATATGGCTCATGGCGTGCATTAGAA
    GAGTTAAAAGAAAATGGTAAAATTAAAGCAATCGGTGTATCTAACTTCGGCGTTGATCGA
    ATTGTAGATTTAGGTATTCACAATCAAATTCAACCACAAGTGAACCAAATAGAAATTAAT
    CCATTCCATCAACAAGAAGAACAAGTTGCAGCACTTCAACAAGAAAATGTTGTGGTAGAA
    GCTTGGGCACCATTTGCAGAAGGTAAAAATCAATTATTCCAAAATCAATTGTTGCAGGCC
    ATTGCAGATAAATACAACAAATCAATTGCACAAGTTATTTTACGCTGGTTAGTAGAACGT
    GACATTGTTGTACTCGCAAAATCAGTGAATCCAGAACGTATGGCTCAAAACTTAGATATA
    TTTGATTTTGAACTTACAGAAGAAGATAAACAACAAATTGCGACATTAGAAGAAAGTAAT
    AGCCAATTTTTCTCACATGCTGATCCAGAGATGATTAAAGCACTTACAAGCAGAGAATTA
    GATGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS11900 [old locus tag: SAUSA300_2159 ]
  • symbol: SAUSA300_RS11900
  • description: aldo/keto reductase
  • length: 282
  • theoretical pI: 4.38261
  • theoretical MW: 32202
  • GRAVY: -0.336525

Function[edit | edit source]

  • reaction:
    EC 1.1.1.218?  ExPASy
    Morphine 6-dehydrogenase Morphine + NAD(P)+ = morphinone + NAD(P)H
  • TIGRFAM:
    voltage-dependent potassium channel beta subunit (TIGR01293; HMM-score: 47.3)
  • TheSEED: see SAUSA300_2159
  • PFAM:
    no clan defined Aldo_ket_red; Aldo/keto reductase family (PF00248; HMM-score: 180.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.105534
    • TAT(Tat/SPI): 0.007155
    • LIPO(Sec/SPII): 0.001178
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEINPFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLVERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]