PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS13315 [old locus tag: SAUSA300_2404 ]
• pan locus tag^?: SAUPAN006018000
• symbol: SAUSA300_RS13315
• pan gene symbol^?: —
• synonym:
• product: FMN-binding glutamate synthase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS13315 [old locus tag: SAUSA300_2404 ]
• symbol: SAUSA300_RS13315
• product: FMN-binding glutamate synthase family protein
• replicon: chromosome
• strand: -
• coordinates: 2588435..2590012
• length: 1578
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2588435..2590012) NCBI
• BioCyc: SAUSA300_RS13315 BioCyc
• MicrobesOnline: see SAUSA300_2404

⊟Phenotype

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⊟DNA sequence

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  ATGACGTTTCTTACAGTCATGCAATTTATAGTTAACATTATCGTTGTAGGATTCATGCTT
  ACGGTTATTGTTATCGGGCTTATTTGGTTAATTAAAGATAAAAGACAATCACAACATAGT
  GTATTAAGGAATTATCCTTTACTAGCACGTATTAGATATATTTCAGAAAAAATGGGACCG
  GAATTACGTCAGTATTTATTTTCTGGGGATAATGAAGGGAAACCTTTTTCACGTAATGAT
  TATAAAAATATCGTTTTGGCTGGAAAATATAACTCTCGTATGACCAGCTTCGGTACTACT
  AAAGATTATCAAGACGGCTTTTACATACAGAACACAATGTTTCCGATGCAACGTAATGAG
  ATTTCAGTAGATAATACAACATTGTTATCAACATTCATTTATAAAATCGCGAATGAGCGT
  TTATTTAGTCGTGAAGAATATCGTGTGCCGACAAAGATTGATCCGTATTACTTAAGTGAT
  GACCATGCAATAAAATTAGGTGAACATTTAAAACATCCATTTATTTTAAAACGTATCGTA
  GGACAATCTGGTATGAGTTATGGCGCTTTAGGAAAAAATGCCATTACAGCTTTATCTAAA
  GGTCTAGCTAAAGCGGGCACTTGGATGAATACAGGTGAAGGTGGCTTATCAGAATATCAT
  TTAAAAGGTAATGGGGATATCATTTTCCAAATTGGTCCCGGTTTATTTGGTGTTCGTGAT
  AAAGAAGGTAATTTTAGTGAAGGTTTATTTAAAGAGGTTGCACAGTTATCTAACGTACGC
  GCATTTGAGCTGAAGTTGGCACAAGGTGCTAAGACTCGTGGTGGTCATATGGAAGCTGAA
  AAGGTAAACGAAGAAATTGCTAAAATCCGAAATGTTGAACCTTATAAAACAATCAATTCA
  CCTAACCGTTACGAATTTATTCATAATGCTGAAGATTTGATTCGTTTCGTCGATCAGTTG
  CAGCAATTAGGTCAAAAACCAGTAGGATTCAAAATTGTAGTAAGCAAAGTTTCAGAAATT
  GAAACACTTGTACGTACGATGGTGGAACTAGATAAGTATCCAAGCTTTATTACGATTGAT
  GGTGGTGAAGGTGGTACTGGTGCAACATTCCAAGAATTACAAGATGGTGTTGGCTTACCG
  CTATTTACAGCTCTACCTATTGTGTCTGGCATGTTAGAAAAATATGGTATTCGAGATAAA
  GTGAAATTGGCGGCATCTGGTAAGTTAGTGACACCAGATAAAATTGCGATTGCACTAGGT
  TTAGGTGCAGATTTTGTAAATATCGCACGTGGGATGATGATTAGTGTCGGTTGTATAATG
  AGTCAACAATGTCACATGAATACGTGTCCTGTAGGTGTTGCAACGACAGATGCGAAGAAA
  GAAAAAGCATTGATTGTTGGAGAAAAGCAATATCGTGTCACAAACTATGTAACAAGTTTG
  CATGAAGGCTTATTCAATATTGCAGCAGCTGTTGGCGTATCCAGTCCTACAGAAATTACT
  GCTGATCATATTGTATATCGAAAAGTCGATGGTGAGTTACAAACGATACATGATTATAAA
  TTAAAACTCATTAGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS13315 [old locus tag: SAUSA300_2404 ]
• symbol: SAUSA300_RS13315
• description: FMN-binding glutamate synthase family protein
• length: 525
• theoretical pI: 9.24714
• theoretical MW: 58439.1
• GRAVY: -0.122857

⊟Function

• TIGRFAM:
  PfaD family protein (TIGR02814; HMM-score: 12.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.9)
• TheSEED: see SAUSA300_2404
• PFAM:
  TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 332.5)
  and 2 more
  Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 18.7)
  IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 15.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002678
  • TAT(Tat/SPI): 0.000389
  • LIPO(Sec/SPII): 0.001608
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446065648 NCBI
• RefSeq: WP_000143503 NCBI
• UniProt: see SAUSA300_2404

⊟Protein sequence

• MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRNYPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKDYQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSDDHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSEYHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGHMEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVVSKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGMLEKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCPVGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRKVDGELQTIHDYKLKLIS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications