PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS13660 [old locus tag: SAUSA300_2463 ]
• pan locus tag^?: SAUPAN006169000
• symbol: SAUSA300_RS13660
• pan gene symbol^?: ddh
• synonym:
• product: lactate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS13660 [old locus tag: SAUSA300_2463 ]
• symbol: SAUSA300_RS13660
• product: lactate dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 2659420..2660412
• length: 993
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2659420..2660412) NCBI
• BioCyc: SAUSA300_RS13660 BioCyc
• MicrobesOnline: see SAUSA300_2463

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAAAAATTATGTTCTTTGGTACGCGTGATTATGAGAAAGAGATGGCATTAAATTGG
  GGGAAAAAGAATAATGTCGAAGTAACTACTTCTAAAGAGCTATTATCAAGTGCTACAGTC
  GATCAATTAAAAGATTACGATGGCGTAACTACAATGCAATTTGGTAAGTTAGAAAATGAC
  GTTTATCCTAAATTAGAATCTTACGGTATTAAACAAATTGCACAACGTACTGCTGGATTT
  GATATGTATGATTTAGATTTAGCTAAAAAACACAATATTGTGATATCTAACGTTCCTAGT
  TATTCACCTGAAACAATTGCAGAGTATTCTGTTTCTATCGCCCTACAATTAGTGCGTCGC
  TTCCCAGATATTGAACGCCGTGTACAAGCACATGATTTTACTTGGCAAGCAGAAATCATG
  TCTAAACCAGTTAAAAATATGACTGTTGCAATTATTGGTACGGGTCGTATCGGTGCTGCT
  ACAGCTAAAATATATGCAGGATTTGGTGCTACAATTACAGCTTATGACGCCTATCCTAAT
  AAAGATTTAGACTTTTTAACTTATAAAGATAGTGTTAAAGAAGCTATTAAAGATGCCGAT
  ATTATTTCTTTACATGTTCCAGCGAACAAAGAAAGCTATCATTTATTCGATAAAGCAATG
  TTTGATCATGTGAAAAAAGGTGCAATCTTAGTTAACGCAGCACGTGGTGCAGTCATCAAT
  ACACCTGATTTAATCGCTGCAGTGAACGATGGTACTTTGTTAGGTGCTGCGATTGATACT
  TATGAAAATGAAGCAGCATACTTCACAAATGACTGGACTAATAAAGACATTGACGATAAA
  ACATTATTAGAGTTAATCGAACATGAAAGAATTTTAGTAACACCACATATTGCTTTCTTC
  TCTGATGAAGCAGTACAAAACCTTGTTGAAGGTGGTTTAAACGCAGCATTATCTGTCATT
  AACACTGGTACATGTGAAACACGTTTAAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS13660 [old locus tag: SAUSA300_2463 ]
• symbol: SAUSA300_RS13660
• description: lactate dehydrogenase
• length: 330
• theoretical pI: 4.89771
• theoretical MW: 36681.4
• GRAVY: -0.193636

⊟Function

• reaction:
  EC 1.1.1.28?  ExPASy
  D-lactate dehydrogenase (R)-lactate + NAD⁺ = pyruvate + NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 154.7)
  and 11 more
  Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 19.7)
  Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 18.3)
  Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 17.1)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 16)
  Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase (TIGR01723; EC 1.12.98.2; HMM-score: 14.7)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 13.8)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 13.5)
  2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 13)
  nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 13)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 12.1)
  3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 11.6)
• TheSEED: see SAUSA300_2463
• PFAM:
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 173.2)
  and 11 more
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 89.8)
  NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 26.5)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 23.8)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 21.9)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 16.2)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.9)
  NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 15.5)
  Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 15.1)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 15)
  Form_Glyc_dh (CL0325) DpaA_N; Dipicolinate synthase subunit A N-terminal domain (PF16924; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009748
  • TAT(Tat/SPI): 0.000599
  • LIPO(Sec/SPII): 0.001647
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446083681 NCBI
• RefSeq: WP_000161536 NCBI
• UniProt: see SAUSA300_2463

⊟Protein sequence

• MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLKDYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYSPETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAATAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDKAMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKDIDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Rex see SAUSA300_2463

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications