From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13770 [old locus tag: SAUSA300_2482 ]
  • pan locus tag?: SAUPAN006202000
  • symbol: SAUSA300_RS13770
  • pan gene symbol?:
  • synonym:
  • product: CHAP domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13770 [old locus tag: SAUSA300_2482 ]
  • symbol: SAUSA300_RS13770
  • product: CHAP domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2679371..2679802
  • length: 432
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCA
    GATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTAT
    AGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTT
    TCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCG
    ACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACAT
    GTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTT
    TATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAAC
    TATATTTACTAA
    60
    120
    180
    240
    300
    360
    420
    432

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS13770 [old locus tag: SAUSA300_2482 ]
  • symbol: SAUSA300_RS13770
  • description: CHAP domain-containing protein
  • length: 143
  • theoretical pI: 10.6057
  • theoretical MW: 16870.2
  • GRAVY: -0.589511

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SAUSA300_2482
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 49.5)
    and 1 more
    no clan defined DUF2280; Uncharacterized conserved protein (DUF2280) (PF10045; HMM-score: 15)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 35 aa
    • SP(Sec/SPI): 0.832771
    • TAT(Tat/SPI): 0.003345
    • LIPO(Sec/SPII): 0.12139
    • Cleavage Site: CS pos: 35-36. SHA-AV. Pr: 0.5959
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQCTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVAVVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]