From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS14150 [old locus tag: SAUSA300_2548 ]
  • pan locus tag?: SAUPAN006317000
  • symbol: SAUSA300_RS14150
  • pan gene symbol?: —
  • synonym:
  • product: MBL fold hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS14150 [old locus tag: SAUSA300_2548 ]
  • symbol: SAUSA300_RS14150
  • product: MBL fold hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2753761..2754447
  • length: 687
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACTAATCAATTTAAAAATAAACAGTCCAAATTACATGACAGTTTAGAATCCATCACA
    AAAAACTTATATGCGACACCTACTTCGGAACTGCCTTTTGATAATCGATTTCTATTTAAA
    TCTTTTATTTTAAAACGTGAAACAGGTAATATTGTTATCTATCATTCCGGACACTTAGGT
    GACTCCCAACAAGACATTGCATCATTAGGTGGTGTTTCAAAAGTATTGATGAATCATGAT
    CATGAATCTATAGGAGGTTCTAATCAAGTTGAAGCCCCTTACTTTATACATGAAAATGAT
    GTGGCTGCACTGAAACATAAGATTTCTGTTCAAAAACAATTTAGTAATCGTGTAATGTTG
    GATAAGGATTTAGAAGTTATTCCCGCGCCTGGACATACACCAGGGACGACACTATTTTTA
    TGGGATGATGGTCATCACCGTTACTTATTTACTGGAGATTTTATATGTTTTGAAGGGAAG
    AGATGGCGTACAGTTATATTAGGTTCAAGTGATAGAGAAAAATCTATTCAAAGTTTAGAG
    ATGGTTAAAGAATTAGATTTTGATGTACTTGTACCTTGGGTTACTATCAAAGATGAACCG
    TTAGTTTATTTTGTAGAAAATGAATATGAAAAACGTGAACAAATACAAAATATTATTGAT
    AGAGTACGTGAGGGCGAGAATAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS14150 [old locus tag: SAUSA300_2548 ]
  • symbol: SAUSA300_RS14150
  • description: MBL fold hydrolase
  • length: 228
  • theoretical pI: 5.98896
  • theoretical MW: 26238.4
  • GRAVY: -0.499561

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 18.3)
    and 1 more
    Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 1 (TIGR04558; HMM-score: 12)
  • TheSEED: see SAUSA300_2548
  • PFAM:
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 28.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004519
    • TAT(Tat/SPI): 0.000387
    • LIPO(Sec/SPII): 0.000357
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFILKRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVAALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGKRWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNIIDRVREGENS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS14150&oldid=102022"