PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14285 [old locus tag: SAUSA300_2568 ]
• pan locus tag^?: SAUPAN006362000
• symbol: SAUSA300_RS14285
• pan gene symbol^?: arcD
• synonym:
• product: arginine-ornithine antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14285 [old locus tag: SAUSA300_2568 ]
• symbol: SAUSA300_RS14285
• product: arginine-ornithine antiporter
• replicon: chromosome
• strand: -
• coordinates: 2779150..2780580
• length: 1431
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2779150..2780580) NCBI
• BioCyc: SAUSA300_RS14285 BioCyc
• MicrobesOnline: see SAUSA300_2568

⊟Phenotype

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⊟DNA sequence

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  ATGAACGAATCAGGAGATAACAAACTCAGTAAATCTTCTTTAATTGGACTAGTTATAGGA
  TCCATGATTGGTGGCGGTGCGTTCAATATAATGTCTGATATGGGCGGTAAAGCCGGTGGA
  TTAGCCATTATTATTGGTTGGATTATTACAGCTATAGGAATGATTTCATTAGCGTTCGTA
  TTTCAAAATTTAACCAATGAACGGCCGGAGCTAGACGGTGGTATTTATAGTTATGCTCAA
  GCAGGATTTGGCGATTTTGTAGGATTTATCAGTGCTTGGGGATATTGGTTCTCAGCGTTT
  TTAGGCAATGTTGCCTATGCAACACTATTGATGTCAGCAGTAGGTAACTTTTTCCCGATT
  TTTAAAGGAGGCAACACATTACCAAGTGTTATTGTCGCCTCGTTACTACTCTGGGGTGTC
  CATTTCTTGATTTTAAAAGGCGTTGAAACAGCAGCATTTATCAATAGTATTGTTACTGTT
  GCAAAGTTAATACCGATTTTACTTGTAATCATATGCATGATAATTGCATTCAATTTTGAC
  ACTTTTAAAACAGGCTTTTTCAGTATGACGTCAGAGGGTGTATTGCCATTTAGTTGGGCG
  AGCACAATGAGCCAAGTTAAAAGTACGATGCTAGTGACAGTTTGGGTGTTTATCGGTATC
  GAAGGTGCAGTAATTTTTTCTAGTAGAGCTAAAAATAAAAAAGATGTAGGTAGTGCCACG
  GTTATAGGACTTATATCAGTTTTAATTATCTATTTCTTATTAACTGTATTAGCTCAAGGC
  GTGATTTTGCAAAATCATATTTCGCAATTAGATTCGCCAAGTATGGCACAGGTGCTTGCA
  ACTATTGTAGGTGGTTGGGGATCTACACTTGTAAATATTGGTTTAATTATTTCGGTACTA
  GGTGCATGGTTAGGATGGACACTGCTTGCTGGTGAATTACCTTTCATTGTTGCAAAAGAT
  GGATTATTTCCAAAATGGTTTGCTAAAGAAAATAAAAATGGAGCACCTGTAAATGCACTG
  CTTATTACCAATATATTAGTACAATTATTTTTAATAAGTATGCTATTTACACAGAGTGCG
  TATCAATTTGCATTTTCACTAGCATCAAGTGCTATTTTATACCCTTACATGTTCAGTGCA
  TTTTACCAAGTTAAATACACTTTAGAGCATCGACAGCAAGCAACTACTAAACAATGGACG
  ATTGGTATCATAGCCTCAATTTATGCTATATGGCTTATATATGCAGCAGGTATCAATTAC
  TTATTATTGACGATGTTACTTTATATTCCAGCTCTTCTTGTTTATACAATCGTTCAAAAG
  AATAATCAGACACGTTTGATTAAATCAGACTATATTCTTTTTATGATTATTATCGTACTT
  GCAGTTATCGGGTTAATTAAGTTATTGATGGGAACGATAAATGTTTTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14285 [old locus tag: SAUSA300_2568 ]
• symbol: SAUSA300_RS14285
• description: arginine-ornithine antiporter
• length: 476
• theoretical pI: 9.50412
• theoretical MW: 51716.2
• GRAVY: 0.907983

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 619.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 568.9)
  and 8 more
  histidine-histamine antiporter (TIGR04298; HMM-score: 140)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 99)
  putrescine-ornithine antiporter (TIGR04299; HMM-score: 81.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 59.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 51.9)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 46.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 24.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines spore germination protein (amino acid permease) (TIGR00912; HMM-score: 21.5)
• TheSEED: see SAUSA300_2568
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 240.8)
  and 4 more
  AA_permease; Amino acid permease (PF00324; HMM-score: 84.2)
  Spore_permease; Spore germination protein (PF03845; HMM-score: 30.4)
  no clan defined YwiC; YwiC-like protein (PF14256; HMM-score: 17.9)
  Gemini_mov; Geminivirus putative movement protein (PF01708; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 13
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012824
  • TAT(Tat/SPI): 0.000841
  • LIPO(Sec/SPII): 0.020508
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 446928586 NCBI
• RefSeq: WP_001005842 NCBI
• UniProt: see SAUSA300_2568

⊟Protein sequence

• MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAIIIGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTVAKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFIGIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMAQVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNGAPVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQQATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSDYILFMIIIVLAVIGLIKLLMGTINVF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: ArcR, ArgR/AhrC, CcpA, Rex see SAUSA300_2568

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications