PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14310 [old locus tag: SAUSA300_2572 ]
• pan locus tag^?: SAUPAN006367000
• symbol: SAUSA300_RS14310
• pan gene symbol^?: aur
• synonym:
• product: peptidase M4 family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14310 [old locus tag: SAUSA300_2572 ]
• symbol: SAUSA300_RS14310
• product: peptidase M4 family protein
• replicon: chromosome
• strand: -
• coordinates: 2784112..2785608
• length: 1497
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2784112..2785608) NCBI
• BioCyc: SAUSA300_RS14310 BioCyc
• MicrobesOnline: see SAUSA300_2572

⊟Phenotype

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⊟DNA sequence

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  ATGGCAGCATTAACCTTGTTGAGCACTTTATCACCAGCAGCATTAGCGATTGATTCAAAA
  AATAAACCAGCTAATTCTGATATTAAATTTGAGGTGACTCAAAAGAGTGATGCGGTCAAA
  GCATTAAAAGAATTGCCTAAATCCGAAAATGTAAAAAATATTTATCAAGATTACGCTGTT
  ACTGATGTAAAAACTGATAAAAAAGGATTTACGCATTATACATTGCAACCGAGTGTTGAT
  GGTGTTCATGCACCTGACAAAGAAGTGAAAGTACACGCAGACAAATCAGGAAAAGTCGTT
  TTAATCAATGGGGATACTGATGCGAAGAAAGTAAAGCCAACGAATAAAGTGACATTAAGT
  AAAGATGACGCAGCCGACAAAGCATTTAAAGCAGTTAAGATTGATAAGAATAAAGCGAAA
  AATCTTAAAGATAAAGTCATTAAAGAAAACAAAGTTGAAATCGATGGTGACAGTAATAAA
  TACGTTTATAATGTTGAGTTAATTACAGTGACACCAGAAATTTCACATTGGAAAGTTAAA
  ATTGATGCTCAAACTGGCGAAATTTTAGAAAAAATGAACTTAGTTAAAGAAGCTGCAGAA
  ACTGGTAAAGGAAAAGGTGTACTTGGCGATACAAAAGATATCAATATCAATAGTATTGAC
  GGTGGATTTAGCCTAGAAGATTTAACGCATCAAGGTAAATTATCAGCATTTAGCTTTAAT
  GATCAAACAGGTCAAGCAACATTGATTACTAATGAAGATGAAAACTTCGTAAAAGATGAG
  CAACGTGCTGGCGTAGATGCAAATTATTACGCTAAACAAACATATGATTATTACAAAGAC
  ACATTTGGTCGTGAATCATATGACAACCAAGGTAGTCCAATTGTTTCATTAACGCATGTT
  AATAACTACGGTGGTCAAGATAACAGAAATAATGCCGCATGGATCGGTGACAAAATGATC
  TATGGTGATGGTGATGGTCGCACATTCACAAGTTTATCGGGTGCAAATGACGTAGTAGCA
  CACGAATTAACACACGGTGTGACACAAGAGACAGCGAACTTAGAATATAAGGACCAGTCA
  GGCGCTCTAAATGAAAGCTTTTCAGATGTTTTTGGATACTTTGTAGATGACGAGGATTTC
  TTAATGGGTGAAGATGTCTACACACCTGGAAAAGAGGGAGACGCTTTACGCAGCATGTCA
  AACCCAGAACAATTTGGTCAACCAGCTCATATGAAAGACTATGTATTCACTGAAAAAGAT
  AATGGTGGCGTACATACGAATTCTGGAATTCCAAATAAAGCAGCTTATAACGTGATTCAA
  GCAATAGGGAAATCTAAATCAGAACAAATTTACTACCGAGCATTAACGGAATACTTAACA
  AGTAATTCAAACTTCAAAGATTGTAAAGATGCATTATACCAAGCGGCTAAAGATTTATAT
  GACGAGCAAACAGCTGAACAGGTGTATGAAGCATGGAATGAAGTAGGCGTGGAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14310 [old locus tag: SAUSA300_2572 ]
• symbol: SAUSA300_RS14310
• description: peptidase M4 family protein
• length: 498
• theoretical pI: 4.74447
• theoretical MW: 54985.4
• GRAVY: -0.701606

⊟Function

• reaction:
  EC 3.4.24.29?  ExPASy
  Aureolysin Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus
• TIGRFAM:
• TheSEED: see SAUSA300_2572
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 176.2)
  Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 142.8)
  and 2 more
  PepSY (CL0320) PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 50.1)
  Cystatin (CL0121) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 47.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 16 aa
  • SP(Sec/SPI): 0.530122
  • TAT(Tat/SPI): 0.013447
  • LIPO(Sec/SPII): 0.060096
  • Cleavage Site: CS pos: 16-17. ALA-ID. Pr: 0.3660
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SAUSA300_2572
• RefSeq: WP_001802650 NCBI
• UniProt: see SAUSA300_2572

⊟Protein sequence

• MAALTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_2572

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications