COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14775 [old locus tag: SAUSA300_pUSA030008 ]
• pan locus tag^?:
• symbol: SAUSA300_RS14775
• pan gene symbol^?: —
• synonym:
• product: IS6 family transposase IS257R2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14775 [old locus tag: SAUSA300_pUSA030008 ]
• symbol: SAUSA300_RS14775
• product: IS6 family transposase IS257R2
• replicon: pUSA03
• strand: +
• coordinates: 8896..9570
• length: 675
• essential: unknown

⊟Accession numbers

• Location: NC_007792 (8896..9570) NCBI
• BioCyc: SAUSA300_RS14775 BioCyc
• MicrobesOnline: see SAUSA300_pUSA030008

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAC
  TATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGGACGTGGTGTA
  AACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAA
  ATTTGGAAGAAAAAGCATAAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATC
  AAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTGATGCAGAGGGACATACATTA
  GATATTTGGTTGCGTAAGCAACGAGATAATCATTCAGCATATGCGTTTATTAAACGTCTC
  ATTAAACAATTTGGTAAACCTCAAAAGGTAATTACAGATCAGGCACCTTCAACGAAGGTA
  GCAATGGCTAAAGTAATTAAAGCTTTTAAACTTAAACCTGACTGCCATTGTACATCGAAA
  TATCTGAATAACCTCATTGAGCAAGATCACCGTCATATTAAAGTAAGAAAGACAAGGTAT
  CAAAGTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATAT
  AAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATC
  ATGCTAGCAAGTTAA
  60
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14775 [old locus tag: SAUSA300_pUSA030008 ]
• symbol: SAUSA300_RS14775
• description: IS6 family transposase IS257R2
• length: 224
• theoretical pI: 10.4686
• theoretical MW: 26813.1
• GRAVY: -0.576339

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_pUSA030008
• PFAM:
  RNase_H (CL0219) DDE_Tnp_IS240; DDE domain (PF13610; HMM-score: 131.5)
  and 8 more
  rve; Integrase core domain (PF00665; HMM-score: 56.2)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 15.9)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 15.6)
  RNase_H (CL0219) DDE_Tnp_IS66; Transposase IS66 family (PF03050; HMM-score: 14.5)
  Lag3_N (CL0720) Cauli_DNA-bind; Caulimovirus DNA-binding protein (PF03310; HMM-score: 13.6)
  HTH (CL0123) HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 13.4)
  NADP_Rossmann (CL0063) OYE-like_second_a-b; OYE-like second alpha/beta domain (PF22620; HMM-score: 13.4)
  HTH (CL0123) HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 8.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.564
  • Cytoplasmic Membrane Score: 0.0015
  • Cell wall & surface Score: 0.0046
  • Extracellular Score: 0.4299
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007803
  • TAT(Tat/SPI): 0.000561
  • LIPO(Sec/SPII): 0.002874
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447028763 NCBI
• RefSeq: WP_001106019 NCBI
• UniProt: see SAUSA300_pUSA030008

⊟Protein sequence

• MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHHSTVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications