COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA010005
• pan locus tag^?:
• symbol: SAUSA300_pUSA010005
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA010005
• symbol: SAUSA300_pUSA010005
• product: hypothetical protein
• replicon: pUSA01
• strand: +
• coordinates: 2749..2922
• length: 174
• essential: unknown

⊟Accession numbers

• Gene ID: 3912717 NCBI
• RefSeq: YP_492683 NCBI
• BioCyc:
• MicrobesOnline: 1291476 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  
  ATGAATAAGTTTTTTTACTTTTTGATTATTTTATTATCTGTATTTTTAATTTTTAATTTT
  GTTAATTCGATTTTATTTGATAATCCTTTTGACTTTTTAGAAAGTTTTATTTATTCATTG
  GTTTTCACATTAATTTATTTAGGTTTAGAGGGTCATAAAAAGAAGAAGGAATAG
  60
  120
  174
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA010005
• symbol: SAUSA300_pUSA010005
• description: hypothetical protein
• length: 57
• theoretical pI: 7.52435
• theoretical MW: 6878.15
• GRAVY: 0.805263

⊟Function

• TIGRFAM:
• TheSEED  :
  • hypothetical protein
• PFAM:
  no clan defined Nodulin_late; Late nodulin protein (PF07127; HMM-score: 13.7)
  GT-C (CL0111) YfhO; Bacterial membrane protein YfhO (PF09586; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Possibly Tat-/Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.083577
  • TAT(Tat/SPI): 0.000277
  • LIPO(Sec/SPII): 0.026539
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87159838 NCBI
• RefSeq: YP_492683 NCBI
• UniProt: A0A0H2XJE9 UniProt

⊟Protein sequence

• MNKFFYFLIILLSVFLIFNFVNSILFDNPFDFLESFIYSLVFTLIYLGLEGHKKKKE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications