COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
• pan locus tag^?:
• symbol: SAUSA300_pUSA030005
• pan gene symbol^?: —
• synonym:
• product: transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
• symbol: SAUSA300_pUSA030005
• product: transposase
• replicon: pUSA03
• strand: +
• coordinates: 5734..6408
• length: 675
• essential: unknown

⊟Accession numbers

• Gene ID: 3912727 NCBI
• RefSeq: YP_492691 NCBI
• BioCyc: see SAUSA300_RS14760
• MicrobesOnline: 1291484 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAC
  TATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGGACGTGGTGTA
  AACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAA
  ATTTGGAAGAAAAAGCATAAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATC
  AAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTGATGCAGAGGGACATACATTA
  GATATTTGGTTGCGTAAGCAACGAGATAATCATTCAGCATATGCGTTTATTAAACGTCTC
  ATTAAACAATTTGGTAAACCTCAAAAGGTAATTACAGATCAGGCACCTTCAACGAAGGTA
  GCAATGGCTAAAGTAATTAAAGCTTTTAAACTTAAACCTGACTGCCATTGTACATCGAAA
  TATCTGAATAACCTCATTGAGCAAGATCACCGTCATATTAAAGTAAGAAAGACAAGGTAT
  CAAAGTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATAT
  AAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATC
  ATGCTAGCAAGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030005 [new locus tag: SAUSA300_RS14760 ]
• symbol: SAUSA300_pUSA030005
• description: transposase
• length: 224
• theoretical pI: 10.4686
• theoretical MW: 26813.1
• GRAVY: -0.576339

⊟Function

• TIGRFAM:
• TheSEED  :
  • Transposase for insertion sequence-like element IS431mec
• PFAM:
  RNase_H (CL0219) DDE_Tnp_IS240; DDE domain (PF13610; HMM-score: 133.2)
  and 9 more
  rve; Integrase core domain (PF00665; HMM-score: 54.7)
  DDE_Tnp_IS66; Transposase IS66 family (PF03050; HMM-score: 18)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.6)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 17.1)
  RNase_H (CL0219) DDE_3; DDE superfamily endonuclease (PF13358; HMM-score: 16.7)
  HTH (CL0123) HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 14.2)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 13.9)
  no clan defined Melibiase_2_C; Alpha galactosidase A C-terminal beta sandwich domain (PF17450; HMM-score: 12.9)
  HEM4; Uroporphyrinogen-III synthase HemD (PF02602; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007803
  • TAT(Tat/SPI): 0.000561
  • LIPO(Sec/SPII): 0.002874
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159860 NCBI
• RefSeq: YP_492691 NCBI
• UniProt: A0A0G2Q3K2 UniProt

⊟Protein sequence

• MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHHSTVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_pUSA030004 > SAUSA300_pUSA030005

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications