COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030007 [new locus tag: SAUSA300_RS14770 ]
• pan locus tag^?:
• symbol: ermC
• pan gene symbol^?: —
• synonym:
• product: rRNA adenine N-6-methyltransferase (macrolide-lincosamide-streptogramin B resistance protein)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030007 [new locus tag: SAUSA300_RS14770 ]
• symbol: ermC
• product: rRNA adenine N-6-methyltransferase (macrolide-lincosamide-streptogramin B resistance protein)
• replicon: pUSA03
• strand: +
• coordinates: 7865..8599
• length: 735
• essential: unknown

⊟Accession numbers

• Gene ID: 3912746 NCBI
• RefSeq: YP_492693 NCBI
• BioCyc: GH3C-2641 BioCyc
• MicrobesOnline: 1291486 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACGAGAAAAATATAAAACACAGTCAAAACTTTATTACTTCAAAACATAATATAGAT
  AAAATAATGACAAATATAAGATTAAATGAACATGATAATATCTTTGAAATCGGCTCAGGA
  AAAGGGCATTTTACCCTTGAATTAGTACAGAGGTGTAATTTCGTAACTGCCATTGAAATA
  GACCATAAATTATGCAAAACTACAGAAAATAAACTTGTTGATCACGATAATTTCCAAGTT
  TTAAACAAGGATATATTGCAGTTTAAATTTCCTAAAAACCAATCCTATAAAATATTTGGT
  AATATACCTTATAACATAAGTACGGATATAATACGCAAAATTGTTTTTGATAGTATAGCT
  GATGAGATTTATTTAATCGTGGAATACGAGTTTGCTAAAAGATTATTAAATACAAAACGC
  TCATTGGCATTATTTTTAATGGCAGAAGTTGATATTTCTATATTAAGTATGGTTCCAAGA
  GAATATTTTCATCCTAAACCTAAAGTGAATAGCTCACTTATCAGATTAAATAGAAAAAAA
  TCAAGAATATCACACAAAGATAAACAGAAGTATAATTATTTCGTTATGAAATGGGTTAAC
  AAAGAATACAAGAAAATATTTACAAAAAATCAATTTAACAATTCCTTAAAACATGCAGGA
  ATTGACGATTTAAACAATATTAGCTTTGAACAATTCTTATCTCTTTTCAATAGCTATAAA
  TTATTTAATAAGTAA
  60
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  735
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030007 [new locus tag: SAUSA300_RS14770 ]
• symbol: ErmC
• description: rRNA adenine N-6-methyltransferase (macrolide-lincosamide-streptogramin B resistance protein)
• length: 244
• theoretical pI: 10.1364
• theoretical MW: 28979.3
• GRAVY: -0.480738

⊟Function

• reaction:
  EC 2.1.1.48?  ExPASy
  Transferred entry: 2.1.1.181, 2.1.1.182, 2.1.1.183 and 2.1.1.184
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 155)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 27.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 23.3)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 23.2)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 17.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 16.6)
• TheSEED  :
  • 23S rRNA (adenine(2058)-N(6))-dimethyltransferase (EC 2.1.1.184) => Erm(C)
• PFAM:
  NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 207.3)
  and 11 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 26.6)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 23.3)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 19.6)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 16.4)
  DOT1; Histone methylation protein DOT1 (PF08123; HMM-score: 16.3)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 15.9)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.6)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 13)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 12.9)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.9)
  DnaA_N (CL0494) DnaA_N; DnaA N-terminal domain (PF11638; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004669
  • TAT(Tat/SPI): 0.000224
  • LIPO(Sec/SPII): 0.000352
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159879 NCBI
• RefSeq: YP_492693 NCBI
• UniProt: A0A0H2XJC5 UniProt

⊟Protein sequence

• MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIYLIVEYEFAKRLLNTKRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNISFEQFLSLFNSYKLFNK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications