COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030022 [new locus tag: SAUSA300_RS14845 ]
• pan locus tag^?:
• symbol: traM
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraM

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030022 [new locus tag: SAUSA300_RS14845 ]
• symbol: traM
• product: transfer complex protein TraM
• replicon: pUSA03
• strand: +
• coordinates: 22645..23037
• length: 393
• essential: unknown

⊟Accession numbers

• Gene ID: 3912748 NCBI
• RefSeq: YP_492708 NCBI
• BioCyc: see SAUSA300_RS14845
• MicrobesOnline: 1291501 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATAAACAAAGTAATATATAGTGGACGTATCGCAACTGATTTAAAATTAAGAAAAAGT
  AAAAATGAAAAGTCTTATGTTTTCTTTAACATAGCTGTACCTAGGCAGTTTGATAATGAT
  AGAACAGATTTTATTAATTGTGTTGTATGGGGAAAACAAGCAGAAAGTTTAGTTGATTAT
  CAAAGTAAAGGTAATCTAATTATTATTGAAGGTTCAACTGAAACATATAAAAAAGACAAT
  ATTAATATGTCTAGATGTTTGGTTGAAAAGACTATCTTTTTAGAAAGCAAAAAAGAAAAC
  AAAAAACATGACGTGTCAGAAGATGACGCTATAAGCCCAGCAGAAGATAGTGGCAACGAT
  AAATCTATCTCTGATGATGACTTACCATTTTAA
  60
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  393
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030022 [new locus tag: SAUSA300_RS14845 ]
• symbol: TraM
• description: transfer complex protein TraM
• length: 130
• theoretical pI: 5.00988
• theoretical MW: 14860.6
• GRAVY: -0.678462

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair single-stranded DNA-binding protein (TIGR00621; HMM-score: 54.4)
• TheSEED  :
  • Single-stranded DNA-binding protein
  DNA Metabolism DNA repair DNA repair, bacterial  Single-stranded DNA-binding protein
  and 2 more
  DNA Metabolism DNA repair DNA repair, bacterial RecFOR pathway  Single-stranded DNA-binding protein
  DNA Metabolism DNA repair DNA repair, bacterial UmuCD system  Single-stranded DNA-binding protein
• PFAM:
  OB (CL0021) SSB; Single-strand binding protein family (PF00436; HMM-score: 67)
  and 2 more
  tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.2)
  tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 15.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004314
  • TAT(Tat/SPI): 0.000204
  • LIPO(Sec/SPII): 0.000703
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159881 NCBI
• RefSeq: YP_492708 NCBI
• UniProt: A0A0H2XIW8 UniProt

⊟Protein sequence

• MINKVIYSGRIATDLKLRKSKNEKSYVFFNIAVPRQFDNDRTDFINCVVWGKQAESLVDYQSKGNLIIIEGSTETYKKDNINMSRCLVEKTIFLESKKENKKHDVSEDDAISPAEDSGNDKSISDDDLPF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traL > traM

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications