COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030032
• pan locus tag^?:
• symbol: nes
• pan gene symbol^?: —
• synonym:
• product: oriT nickase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030032
• symbol: nes
• product: oriT nickase
• replicon: pUSA03
• strand: -
• coordinates: 33233..34144
• length: 912
• essential: unknown

⊟Accession numbers

• Gene ID: 3912757 NCBI
• RefSeq: YP_492718 NCBI
• BioCyc:
• MicrobesOnline: 1291511 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
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  901
  
  ATGGCAATGTACCATTTTCAAAATAAGTTTGTATCAAAAGCAAATGGACAAAGTGCAACT
  GCAAAAAGTGCCTATAATAGCGCTAGTAGGATTAAAGATTTTAAGGAAAACGAATTTAAA
  GATTACTCAAATAAGCAATGTGATTATAGTGAAATTTTATTACCTAATAATGCTGATGAT
  AAGTTTAAAGATAGAGAATATTTGTGGAATAAAGTTCATGATGTTGAGAACAGAAAAAAT
  AGTCAAGTTGCACGAGAAATTATAATTGGTTTGCCGAATGAATTTGATCCAAATAGTAAT
  ATTGAATTAGCAAAAGAATTTGCTGAATCCTTATCTAATGAAGGAATGATTGTTGATCTA
  AATATTCATAAAATAAATGAAGAAAATCCTCACGCACATTTATTATGTACATTACGTGGT
  TTAGATAAAAATAATGAATTTGAACCTAAGCGAAAGGGCAATGATTATATTAGAGATTGG
  AATACAAAAGAAAAACATAATGAATGGCGAAAACGGTGGGAAAATGTTCAAAATAAACAC
  CTAGAAAAGAATGGTTTTTCTGTTAGAGTTTCTGCTGATTCATATAAAAATCAAAATATA
  GATTTAGAGCCAACAAAAAAAGAAGGTTGGAAAGCACGAAAATTTGAAGATGAAACAGGA
  AAAAAATCTTCAATATCGAAACATAATGAAAGTATTAAAAAGAGAAATCAGCAAAAAATT
  GATAAGATGTTTAATGAAGTAGATGATATGAAATCTCACAAATTAAATGCTTTTAGTTAT
  ATGAATAAATCAGATTCTACTACATTAAAAAATATGGCCAAAGATTTAAAAATTTATGTA
  ACACCTATAAATATGTATAAGGAAAATGAAAGACTATATGATTTAAAACAAAAAAACTTC
  ATTAATCACTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030032
• symbol: Nes
• description: oriT nickase
• length: 303
• theoretical pI: 9.50844
• theoretical MW: 35854.9
• GRAVY: -1.21848

⊟Function

• TIGRFAM:
  Ti-type conjugative transfer relaxase TraA (TIGR02768; HMM-score: 204.6)
• TheSEED  :
  • oriT nickase Nes
• PFAM:
  Rep (CL0169) MobA_MobL; MobA/MobL family (PF03389; HMM-score: 188.1)
  and 2 more
  YqgF (CL0580) RuvX; Holliday junction resolvase (PF03652; HMM-score: 13.1)
  Peptidase_MA (CL0126) DUF2268; Predicted Zn-dependent protease (DUF2268) (PF10026; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.67
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029606
  • TAT(Tat/SPI): 0.003534
  • LIPO(Sec/SPII): 0.008187
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159854 NCBI
• RefSeq: YP_492718 NCBI
• UniProt: A0A0H2XIT9 UniProt

⊟Protein sequence

• MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYKENERLYDLKQKNFINH

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications