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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00030 [old locus tag: SAP008 ]
  • pan locus tag?:
  • symbol: SA_RS00030
  • pan gene symbol?: —
  • synonym:
  • product: quinone oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00030 [old locus tag: SAP008 ]
  • symbol: SA_RS00030
  • product: quinone oxidoreductase
  • replicon: pN315
  • strand: -
  • coordinates: 5905..6876
  • length: 972
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_003140 (5905..6876) NCBI
  • BioCyc: SA_RS00030 BioCyc
  • MicrobesOnline: see SAP008

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTCAATGCGGTAGTAGTGAGAAAAAGTGCATCAAATGTTAGTTATAAAGTAGAAAAA
    GTTGATGAATCTCATTTATCGAAGGGTGATGTATTAGTAAAAGTGGTATACTCTTCAATA
    AATTATAAGGATATGCTAGCTTTACAATATAAAGGCGGTGTAATTAGAGATTATCCTATG
    ATTCCTGGTATAGATTTTGCAGGTATTGTTGAATCAAGTTCGAATGATAAGTTTAAAGAA
    GGCGATAATGTATTAGTTACTGGTTATGATGTTGGCGTGACACATACAGGTGGATTTTCA
    GAATATGCACAAGTGCCTGGAGAGTGGATTGTTCCTATTCCCCAAGAAATGTCTATTAAA
    GAGTCTATGGTATATGGCACAGCCGGTTTTACGGCAGCATTATCAATTTTTGAACTAGAA
    AAATATGGAATGAATATCAAATCACAACCTGAAATTTTAGTGTCTGGGGCTACAGGTGGT
    GTGGGCAGTGTTGCTATACAAATTTTACATAAAATTGGATATGAAAATATCACTGCTATC
    GTAAGAAAAGAAAAGCAAATAGAAATCGCAAAAAAATTAGGTGCAAAACAAGTAATAATA
    ATTGAAGATGATAATAAACCGCTTAAAAAAGGTATTTTTGATTATTTCTTAGATACAGTG
    GGGAGTAATGTAACATTATACGGTTTAAAACGTCTAAATTATCAAGGTGTTGCTACTGTA
    TGTGGAAATGTAGCAGGAAATTCACTTAACGCTAATATATTACCGTTTATTCTTAGAGGT
    ATTAAATTAATTGGCATTGATTCTGTATATGTTGATCATAATATCCGTGAAGATATATGG
    AGTAAGTTAGCAAATGAATGGAATATAGGAAATTCACTTCTATACAATGAAATTGGATTT
    GAAGAATTCTATAAAACGATAGATCAGCTTTTAAAAGGACAACATTTAGGAAGAACTATT
    TTAAAAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS00030 [old locus tag: SAP008 ]
  • symbol: SA_RS00030
  • description: quinone oxidoreductase
  • length: 323
  • theoretical pI: 6.69127
  • theoretical MW: 35597.7
  • GRAVY: 0.00495356

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 425.7)
    and 15 more
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 98.9)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 67.8)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 56.5)
    Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 42.5)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 42.3)
    Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 39.8)
    Metabolism Energy metabolism Fermentation S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 39.8)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 36.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 32.5)
    Cellular processes Cellular processes Detoxification S-(hydroxymethyl)mycothiol dehydrogenase (TIGR03451; EC 1.1.1.306; HMM-score: 29.5)
    putative phosphonate catabolism associated alcohol dehydrogenase (TIGR03366; HMM-score: 23.5)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 21.9)
    Metabolism Central intermediary metabolism Other long-chain fatty acyl-ACP reductase (aldehyde-forming) (TIGR04058; EC 1.1.1.-; HMM-score: 12.6)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02822; EC 1.-.-.-; HMM-score: 12.3)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 11.2)
  • TheSEED: see SAP008
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 53.1)
    and 6 more
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 31.7)
    NADP_Rossmann (CL0063) DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 19.3)
    NmrA; NmrA-like family (PF05368; HMM-score: 16.5)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 16.4)
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 15.9)
    NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005133
    • TAT(Tat/SPI): 0.000222
    • LIPO(Sec/SPII): 0.000963
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDMLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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