COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00040 [old locus tag: SAP010 ]
• pan locus tag^?:
• symbol: SA_RS00040
• pan gene symbol^?: —
• synonym:
• product: class A beta-lactamase BlaZ

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00040 [old locus tag: SAP010 ]
• symbol: SA_RS00040
• product: class A beta-lactamase BlaZ
• replicon: pN315
• strand: -
• coordinates: 7945..8790
• length: 846
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (7945..8790) NCBI
• BioCyc: SA_RS00040 BioCyc
• MicrobesOnline: see SAP010

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGAAAAAGTTAATATTTTTAATTGTAATTGCTTTAGTTTTAAGTGCATGTAATTCAAAC
  AGTTCACATGCCAAAGAGTTAAATGATTTAGAAAAAAAATATAATGCTCATATTGGTGTT
  TATGCTTTAGATACTAAAAGTGGTAAGGAAGTAAAATTTAATTCAGATAAGAGATTTGCC
  TATGCTTCGACTTCAAAAGCGATAAATAGTGCTATTTTGTTAGAACAAGTACCTTATAAT
  AAGTTAAATAAAAAAGTACATATTAACAAAGATGATATAGTTGCTTATTCTCCTATTTTA
  GAAAAATATGTAGGAAAAGATATCACTTTAAAAGAACTTATTGAGGCTTCAATGACATAT
  AGTGATAATACAGCAAACAATAAAATTATAAAAGAAATCGGTGGAATCAAAAAAGTTAAA
  CAACGTCTAAAAGAACTAGGAGATAAAGTAACAAATCCAGTTAGATATGAGATAGAATTA
  AATTACTATTCACCAAAGAGCAAAAAAGATACTTCAACGCCTGCTGCTTTCGGCAAGACT
  TTAAATAAACTTATCGCAAATGGAAAATTAAGCAAAAAAAATAAAAATTTCTTACTTGAT
  TTAATGTTTAATAATAAAAACGGAGACACTTTAATTAAAGATGGTGTTCCAAAAGACTAT
  AAGGTTGCTGATAAAAGTGGTCAAGCAATAACATATGCTTCTAGAAATGATGTTGCTTTT
  GTTTATCCTAAGGGCCAATCTGAACCTATTGTTTTAGTCATTTTTACGAATAAAGACAAT
  AAAAGTGATAAGCCAAATGATAAGTTGATAAGTGAAACCGCCAAGAGTGTAATGAAGGAA
  TTTTAA
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⊟Protein

⊟General

• locus tag: SA_RS00040 [old locus tag: SAP010 ]
• symbol: SA_RS00040
• description: class A beta-lactamase BlaZ
• length: 281
• theoretical pI: 10.1468
• theoretical MW: 31453.1
• GRAVY: -0.492883

⊟Function

• reaction:
  EC 3.5.2.6?  ExPASy
  Beta-lactamase A beta-lactam + H₂O = a substituted beta-amino acid
• TIGRFAM:
• TheSEED: see SAP010
• PFAM:
  Beta-lactamase (CL0013) Beta-lactamase2; Beta-lactamase enzyme family (PF13354; HMM-score: 127)
  and 3 more
  Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 77.7)
  Peptidase_S11; D-alanyl-D-alanine carboxypeptidase (PF00768; HMM-score: 17.3)
  no clan defined FadA; Adhesion protein FadA (PF09403; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.53
  • Cellwall Score: 8.75
  • Extracellular Score: 0.7
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 16 aa
  • SP(Sec/SPI): 0.033339
  • TAT(Tat/SPI): 0.000123
  • LIPO(Sec/SPII): 0.966298
  • Cleavage Site: CS pos: 16-17. LSA-CN. Pr: 0.9681
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446655939 NCBI
• RefSeq: WP_000733285 NCBI
• UniProt: see SAP010

⊟Protein sequence

• MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALDTKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BlaI see SAP010

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications