COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00045 [old locus tag: SAP011 ]
• pan locus tag^?:
• symbol: SA_RS00045
• pan gene symbol^?: —
• synonym:
• product: beta-lactam sensor/signal transducer BlaR1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00045 [old locus tag: SAP011 ]
• symbol: SA_RS00045
• product: beta-lactam sensor/signal transducer BlaR1
• replicon: pN315
• strand: +
• coordinates: 8897..10654
• length: 1758
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (8897..10654) NCBI
• BioCyc: SA_RS00045 BioCyc
• MicrobesOnline: see SAP011

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1741
  
  ATGGCTAAATTACTAATAATGAGCGTAGTAAGCTTTTGTTTTATATTTCTATTGTTAGTA
  TTTTTTAGGTACATATTAAAACGCTATTTTAATTATAGTTTAAATTATAAAGTTTGGTAT
  CTAACTATGCTTGCTGGATTAATTCCTTTTATCCCAATTAAATTCTCTTTTATAAAATTT
  AATAATGTGAATAATCAAGCGCCCACAGTTGAAAGTAAGTCACACGATTTGAACCATAAC
  ATAAATACCACCAAACCTATTCAAGAGTTCACAACAGATATCCATAAGTTTAATTGGGAT
  TCAATTGATAATATCTGCACAGTTATTTGGATAGTTTTAGTTATTATTTTAAGTTTTAAA
  TTTTTGAAAGCCTTATTATATCTTAAATATTTAAAGAAACAGTCACTTTATCTAAACGAA
  AATGAAAAAAATAAAATAGATACGATACTTTTCAACCATCAATATAAAAAAAATATTGTG
  ATTCGAAAAGCAGAGGCTATTCAATCTCCAATAACTTTTTGGTATGGGAAATATATTATT
  TTGATTCCTAGTTCATATTTTAAAAGTGTAATTGATAAAAGACTAAAATATATAATACTG
  CATGAATATGCTCATGCTAAAAATAGAGATACTTTACATTTAATTATCTTTAATATCTTC
  TCTATTATTATGAGTTACAATCCATTAGTACATATTGTAAAAAGAAAGATAATTCATGAC
  AATGAAGTAGAAGCCGATAGATTTGTCCTTAATAATATTAACAAAAATGAATTTAAAACT
  TATGCGGAATCTATTATGGACTCCGTATTAAAAACCCCATTTTCCAACAAAAATATATTA
  AGCCATTCATTTAATGGTAAAAAATCATTACTCAAAAGTAGATTAATTAATATAAAAGAA
  GCCGATCTGAAAAAGCAGTCAAAATTAATTCTAATTTTTATTTGTATTTTTACTTTTTTT
  ATCATGATAATTCAAAGCCAGTTTTTAATGGGGCAATCCTTAACTGATTATAATTATAAA
  AAGCCCTTACAAAGCGATTACCAAATTTTAGATGAAAGTAAAAATTTTGGTTCAAATAGT
  GGATCTTTCGTTATGTACAGCATGAAGAAAGACAAATACTATATTTATAATGAAAAAGAA
  AGTAGAAAAAGATATTCTCCTGATTCAACTTACAAAATTTATTTAGCACTGTTTGGACTT
  GACCGTCATATTATAAGTGATAAAAATTCACGTATGTCATGGAATCATAAACATTATCTT
  TTCGAGTCTTGGAATAAGGAACAAGATTTAAATACAGCAATGCAAAATTCAGTTAATTGG
  TACTTCGAACGTATTAGTAATCAAATACCAAAGAACTATACTGCGGCTCAACTCAAGCAA
  TTAAATTATGGTAATGAAAATTTGGGAAGTTATAAAAGCTATTGGATGGAAGATAGTTTG
  AAAATATCTAATCTTGAACAAGTAATAGTTTTTAAAAATATGATGGAACAAAATAACCAT
  TTTAGTAAAAAAGCAAAGAATCAATTATCTTCTTCATTATTGATTAAGAAAAATGAAAAG
  TATGAACTGTATGGGAAAACAGGTACAGGTATAGTAAACGGGAAGTATAATAATGGGTGG
  TTTGTAGGTTACGTAATTACAAATCATGATAAGTATTATTTTGCTACACATTTATCAGAT
  GGAAACCCGTCTGGGAAAAATGCTGAATTAATAAGTGAAAAAATATTAAAAGGAATGGGT
  GTTTTAAATGACCAATAA
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  1758
  

⊟Protein

⊟General

• locus tag: SA_RS00045 [old locus tag: SAP011 ]
• symbol: SA_RS00045
• description: beta-lactam sensor/signal transducer BlaR1
• length: 585
• theoretical pI: 10.0095
• theoretical MW: 69051.6
• GRAVY: -0.281709

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 26.3)
• TheSEED: see SAP011
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M56; BlaR1 peptidase M56 (PF05569; HMM-score: 309.4)
  and 2 more
  Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 135.5)
  Peptidase_MA (CL0126) Peptidase_M48; Peptidase family M48 (PF01435; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001417
  • TAT(Tat/SPI): 0.000254
  • LIPO(Sec/SPII): 0.008956
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 447019125 NCBI
• RefSeq: WP_001096381 NCBI
• UniProt: see SAP011

⊟Protein sequence

• MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLAGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNDQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BlaI see SAP011

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications