COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00060 [old locus tag: SAP014 ]
• pan locus tag^?:
• symbol: SA_RS00060
• pan gene symbol^?: —
• synonym:
• product: transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00060 [old locus tag: SAP014 ]
• symbol: SA_RS00060
• product: transposase
• replicon: pN315
• strand: +
• coordinates: 11853..13295
• length: 1443
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (11853..13295) NCBI
• BioCyc: SA_RS00060 BioCyc
• MicrobesOnline: see SAP014

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAAAAACAAGGAGAAGTATCTAACTAATTTTTCTGAAGCCAAACGTAAAAAAGCTACT
  CAAAAGTACAATATTATAAAACCTTTTATCCTAGGCGAACAATCTTTATCAAGTATTTCT
  AAAAGTAAAGGGATTGCGTTAAGCACCCTTTATAGATGGAATAAGTCATATAAAGAACAA
  GGACTTAAAGGACTAATCTATGCGACAAAAGCTGATAAAGGAACACGTAAAATAGAACCA
  AAGATAATTGATGAAATTGAACGCCTTGCGCTTATGAATAAAAGAAATAGTATAGCCACA
  ATTCATAGGAAAACCACTAATTACTGTAAAGAAAATAATTTTGATATACCAAGTTATAAA
  CAAATTTATAGTGTTATTAAAGCGATGCCTAAGTCTGTTATTGATTTCTCTCATCAGGGT
  GAAAAATACTATCAAAATAAGTACGATTTAATACAAATTAGAGAATCCTCGAGACCCAAT
  GAAATATGGCAAGCAGACCATACTTTGCTAGATATCTTTATATTAGACCAAAAAGGTAAT
  ATCAATAGACCATGGCTAACTATTATTATGGATGACTATAGTCGTGCAATTGCAGGCTAT
  TTCTTAAGTTTTGATGCCCCCAATGCTCAAAACACAGCATTAACACTACACCAAGCAATA
  TGGAATAAAAATGATACCAATTGGCCAGTGTGTGGTATACCCGAAAAATTCTACACAGAT
  CATGGAAGTGACTTTACTTCACATCATATGGAACAAGTCGCTATTGATTTGAAAATTAAC
  TTGATGTTTTCGAAAGTTGGTGTTCCTCGTGGTCGTGGAAAAATAGAACGATTCTTTCAG
  ACAGTTAATCAAACTTTCTTAGAACAACTGCCTGGATATATAAACAATAATGATACTCCT
  AGTAATCTAATAAATTTTCACAATTTTGAAGAAAAATTACGTTACTTTTTAATTGAAGAT
  TATAATCAAAAAGAACATAGTACTATTCATACCACGCCTATCAGTCGTTGGAATAGTAAT
  CACTTTTTCCCTAATATGCCGAGTAGCTTAGAACAACTTGATTTATTATTGTTGGAAATA
  CCTAAATCTAGAAAAGTTCATTCAGATGGCATTCATTTTCAAGGTTTTCGATACAGTAAT
  ACTAATCTAGCAGCTTATGTAGGTGAATATGTACTGATTCGTTATAACCCAAATGATATG
  GCTGAAATACGTGTGTTTTATAGAGATGAATTTCTTTGTACTGCTATATGTCCAGAAATA
  GCTGATTACTCCATAGATATAAAAGACATACAGCACGCTCGTAGTCAGAGAAGAAAACAC
  TTAAAACAAAATATTGCTAGTCCAAGTACCACGGAATTAATTAAGGAAGAAAAAAATTGT
  GGTTATTCACCTCAAGAAACGACTAAAAACGTCAAAAAATTAAAGAGGTATCGTAATGAC
  TAA
  60
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  360
  420
  480
  540
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  720
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  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1443
  

⊟Protein

⊟General

• locus tag: SA_RS00060 [old locus tag: SAP014 ]
• symbol: SA_RS00060
• description: transposase
• length: 480
• theoretical pI: 9.64625
• theoretical MW: 56158.5
• GRAVY: -0.68625

⊟Function

• TIGRFAM:
• TheSEED: see SAP014
• PFAM:
  RNase_H (CL0219) rve; Integrase core domain (PF00665; HMM-score: 93.6)
  and 11 more
  no clan defined Mu-transpos_C; Mu transposase, C-terminal (PF09299; HMM-score: 61.2)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 23.2)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 20.2)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 19.7)
  HTH_Tnp_1; Transposase (PF01527; HMM-score: 16.4)
  RNase_H (CL0219) rve_3; Integrase core domain (PF13683; HMM-score: 15.5)
  HTH (CL0123) HTH_Tnp_Mu_2; Mu DNA binding, I gamma subdomain (PF09039; HMM-score: 15.3)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 15)
  LZ_Tnp_IS481; leucine-zipper of insertion element IS481 (PF13011; HMM-score: 14.5)
  ADP-ribosyl (CL0084) Pertussis_S1; Pertussis toxin, subunit 1 (PF02917; HMM-score: 12.8)
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022751
  • TAT(Tat/SPI): 0.001209
  • LIPO(Sec/SPII): 0.00348
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446713370 NCBI
• RefSeq: WP_000790712 NCBI
• UniProt: see SAP014

⊟Protein sequence

• MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKGIALSTLYRWNKSYKEQGLKGLIYATKADKGTRKIEPKIIDEIERLALMNKRNSIATIHRKTTNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPNEIWQADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQAIWNKNDTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIERFFQTVNQTFLEQLPGYINNNDTPSNLINFHNFEEKLRYFLIEDYNQKEHSTIHTTPISRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKVHSDGIHFQGFRYSNTNLAAYVGEYVLIRYNPNDMAEIRVFYRDEFLCTAICPEIADYSIDIKDIQHARSQRRKHLKQNIASPSTTELIKEEKNCGYSPQETTKNVKKLKRYRND

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications