COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00075 [old locus tag: SAP017 ]
• pan locus tag^?:
• symbol: SA_RS00075
• pan gene symbol^?: —
• synonym:
• product: arsenical efflux pump membrane protein ArsB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00075 [old locus tag: SAP017 ]
• symbol: SA_RS00075
• product: arsenical efflux pump membrane protein ArsB
• replicon: pN315
• strand: +
• coordinates: 14421..15710
• length: 1290
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (14421..15710) NCBI
• BioCyc: SA_RS00075 BioCyc
• MicrobesOnline: see SAP017

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGACTATTTTAGCAATTGTAATTTTTCTTTTAACTTTAACCTTTGTGATTTGGCAACCA
  AAAGGTTTAGATATTGGTATTACAGCTTTAATTGGAGCGGTTGTTGCTATCATTACAGGA
  GTCGTAAGTCTTTCTGATGTATTAGAAGTAACAGGTATTGTTTGGAATGCAACTTTAACT
  TTTGTGGCTGTTATTCTTATTTCATTAATATTAGATGAAATTGGTTTTTTTGAATGGTCT
  GCGATACATATGGTCAAGGCTTCAAACGGTAATGGCTTAAAAATGTTTGTTTTTATTATG
  TTACTTGGGGCAATTGTAGCAGCATTTTTCGCAAATGATGGTGCAGCTTTAATCTTAACG
  CCTATTGTATTAGCGATGGTAAGGAATCTAGGATTTAATCAAAAAGTGATTTTCCCCTTT
  ATTATTGCCAGTGGTTTTATTGCTGATACTACATCACTTCCCTTAATTGTAAGTAACTTA
  GTTAATATCGTTTCTGCAGATTACTTCGATATTGGATTTATTGAATATTTTAGCCGAATG
  ATCATTCCTAATATATTCTCTCTGATTGCTAGTATTCTCGTTTTATGGTTATATTTCAGA
  AAATCCATACCTAAAACGTTCGATACAGAAAATCTATCAGATCCTAAAAGTGCAATTAAA
  GATTCTAAGTTATTTAAGCTTTCATGGATAGTATTAGCAGTACTACTCGTTGGATATCTT
  GTTAGCGAGTTTATCCAAATTCCTGTATCAATTATTGCTGGTATTATTGCTTTTATCTTT
  GTAATATTAGCTCGTAAATCTAAAGCAGTTCATACAAAACAAGTGATTAAAGGTGCACCA
  TGGAATATCGTTGTATTCTCTATTGGTATGTATCTTGTTGTGTTTGGACTAAAAAATGTA
  GGTATTACAACGGTCCTTGGGGATGTTTTAACAAACATTTCAAATTATGGGTTATTTAGC
  AGCATCATGGGTATGGGCTTTATAGCTGCTTTCTTATCTTCGATTATGAACAACATGCCA
  ACTGTTTTAATAGATGCAATAGCGATTGGCCAATCCAGTGCTACAGGAATATTAAAAGAA
  GGCATGGTTTATGCGAATGTCATAGGTTCTGATTTAGGACCTAAAATTACGCCAATTGGT
  TCTTTAGCAACATTATTGTGGCTACATGTCTTAACACAAAAAGGTGTGAAGATTTCGTGG
  GGAACATACTTTAAAACTGGAATTATCATCACTATTCCAGTCCTATTTGTAACACTCTTA
  GGTTTATACCTTACACTAATCATATTTTAA
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⊟Protein

⊟General

• locus tag: SA_RS00075 [old locus tag: SAP017 ]
• symbol: SA_RS00075
• description: arsenical efflux pump membrane protein ArsB
• length: 429
• theoretical pI: 9.55902
• theoretical MW: 46469.7
• GRAVY: 1.15291

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Detoxification arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
  Metabolism Transport and binding proteins Other arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
  and 2 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
  Metabolism Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
• TheSEED: see SAP017
• PFAM:
  IT (CL0182) ArsB; Arsenical pump membrane protein (PF02040; HMM-score: 651.9)
  and 2 more
  CitMHS; Citrate transporter (PF03600; HMM-score: 96)
  Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 40.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016996
  • TAT(Tat/SPI): 0.00013
  • LIPO(Sec/SPII): 0.011195
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 446075776 NCBI
• RefSeq: WP_000153631 NCBI
• UniProt: see SAP017

⊟Protein sequence

• MTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVSLSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIMLLGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSNLVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKSAIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQVIKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLSSIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVLTQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: ArsR see SAP017

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications