From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00250 [old locus tag: SA0019 ]
  • pan locus tag?: SAUPAN000034000
  • symbol: SA_RS00250
  • pan gene symbol?: walH
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00250 [old locus tag: SA0019 ]
  • symbol: SA_RS00250
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 27467..28801
  • length: 1335
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (27467..28801) NCBI
  • BioCyc: SA_RS00250 BioCyc
  • MicrobesOnline: see SA0019

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATAATAAGGAACATATTAAATCTGTCATTTTAGCGCTACTCGTCTTGATGAGTGTC
    GTATTGACATATATGGTATGGAACTTTTCTCCTGATATTGCAAATGTCGACAATACAGAT
    AGTAAGAAGAGTGAAACGAAACCTTTAACGACACCTATGACAGCCAAAATGGATACAACT
    ATTACGCCATTTCAGATTATTCATTCGAAAAATGATCATCCAGAAGGTACGATTGCGACG
    GTATCTAATGTGAATAAACTGACGAAACCTTTGAAAAATAAAGAAGTGAAGTCCGTGGAA
    CATGTTCGTCGTGATCATAACTTGATGATTCCGGATTTAAGCAGTGATTTTACATTATTC
    GATTTTACGTATGATTTACCGTTATCAACATATCTTGGCCAAGTACTGAACATGAATGCG
    AAAGTACCAAATCATTTCAATTTCAATCGTTTAGTCATAGATCATGATGCTGATGATAAT
    ATCGTGCTTTATGCTATAAGTAAAGACCGCCACGATTACGTAAAATTAACAACTACAACG
    AAAAATGATCATTTTTTAGATGCATTAGCAGCAGTGAAAAAAGATATGCAACCATACACA
    GATATCATCACAAACAAAGATACAATTGATCGTACGACGCATGTTTTTGCACCAAGTAAA
    CCTGAAAAGTTAAAAACATATCGCATGGTATTTAACACGATTAGTGTTGAGAAAATGAAT
    GCTATACTATTTGACGATTCAACCATCGTTCGTAGTTCAAAGAGTGGTGTTACAACCTAC
    AACAATAATACAGGTGTCGCAAACTATAACGATAAAAATGAAAAATATCATTATAAAAAC
    CTGTCCGAAGATGAAGCAAGTTCCAGCAAAATGGAAGAAACGATTCCAGGAACCTTTGAT
    TTTATTAATGGTCATGGTGGTTTCTTAAACGAAGACTTCAGATTGTTTAGTACGAATAAT
    CAGTCAGGCGAGTTAACATATCAACGTTTCCTTAACGGTTATCCAACGTTTAATAAAGAA
    GGCTCTAATCAAATTCAAGTCACTTGGGGTGAAAAAGGCGTCTTTGATTATCGTCGTTCG
    TTATTGCGCACCGACGTTGTGTTAAATAGTGAGGATAATAAAACGTTGCCGAAATTAGAG
    TCTGTACGTTCAAGCTTAGCGAACAATAGTGATATTAATTTTGAAAAAGTAACAAACATC
    GCTATCGGTTACGAAATGCAGGATAACTCAGATCATAATCACATTGAAGTGCAGATTAAC
    AGTGAACTCGTACCGCGTTGGTATGTAGAATATGATGGTGAATGGTATGTTTATAACGAT
    GGGAGGCTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1335

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS00250 [old locus tag: SA0019 ]
  • symbol: SA_RS00250
  • description: hypothetical protein
  • length: 444
  • theoretical pI: 5.74071
  • theoretical MW: 50899.6
  • GRAVY: -0.590766

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 16.7)
    Cellular processes Cellular processes Sporulation and germination sporulation protein YunB (TIGR02832; HMM-score: 15.4)
  • TheSEED: see SA0019
  • PFAM:
    YycI_YycH (CL0285) YycH; YycH protein (PF07435; HMM-score: 448.3)
    and 1 more
    no clan defined Gly_acyl_tr_N; Aralkyl acyl-CoA:amino acid N-acyltransferase (PF06021; HMM-score: 10.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.534722
    • TAT(Tat/SPI): 0.001371
    • LIPO(Sec/SPII): 0.014319
    • Cleavage Site: CS pos: 36-37. ANV-DN. Pr: 0.1601
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKSETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHVRRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDNIVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAPSKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKYHYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGYPTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDINFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]