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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00495 [old locus tag: SA0070 ]
  • pan locus tag?: SAUPAN000457000
  • symbol: SA_RS00495
  • pan gene symbol?: kdpB
  • synonym:
  • product: potassium-transporting ATPase subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00495 [old locus tag: SA0070 ]
  • symbol: SA_RS00495
  • product: potassium-transporting ATPase subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 78811..80832
  • length: 2022
  • essential: no DEG

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (78811..80832) NCBI
  • BioCyc: SA_RS00495 BioCyc
  • MicrobesOnline: see SA0070

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGAAACTACTAAAATATTTGAATCACATTTGGTCAAACAGGCTCTAAAAGACAGT
    GTATTGAAGCTCTATCCTGTTTATATGATTAAAAATCCGATTATGTTTGTTGTAGAAGTG
    GGCATGCTGCTTGCCTTAGGATTAACCATTTATCCGGATTTATTTCACCAAGAAAGTGTA
    TCACGGCTATATGTGTTCAGTATCTTTATCATATTATTACTGACACTTGTCTTTGCGAAC
    TTCTCTGAAGCATTAGCTGAAGGTCGCGGTAAAGCACAAGCCAACGCTTTACGCCAAACA
    CAAACTGAAATGAAGGCACGTCGTATTAAACAAGACGGCAGTTATGAAATGATTGACGCT
    AGTGACCTGAAAAAAGGACATATCGTACGTGTCGCGACAGGTGAACAAATCCCAAATGAC
    GGTAAAGTTATTAAGGGCCTCGCAACAGTGGATGAATCTGCGATTACAGGTGAATCTGCA
    CCTGTAATCAAAGAAAGCGGTGGAGATTTCGATAATGTAATTGGAGGAACTTCTGTAGCT
    TCAGACTGGTTAGAAGTTGAGATTACTTCAGAACCAGGTCATTCATTTTTAGATAAAATG
    ATTGGTTTGGTTGAAGGGGCTACAAGAAAGAAAACACCTAATGAAATTGCGTTATTTACT
    TTATTGATGACATTAACGATTATCTTCTTGGTCGTTATTTTAACGATGTATCCATTGGCG
    AAATTCCTGAATTTCAATTTATCCATTGCGATGCTGATTGCTTTGGCTGTGTGTTTAATT
    CCAACAACCATTGGGGGATTATTATCGGCTATAGGGATTGCAGGGATGGATCGTGTGACA
    CAGTTTAATATCTTGGCTAAAAGCGGACGTTCTGTAGAGACTTGTGGTGATGTGAATGTC
    TTGATTTTAGATAAAACAGGTACCATTACCTACGGCAACCGTATGGCAGATGCGTTTATT
    CCGGTGAAATCATCAAGCTTTGAACGTTTAGTTAAAGCGGCCTATGAAAGTTCTATCGCA
    GATGACACACCAGAGGGACGTAGTATTGTGAAATTAGCTTATAAACAACATATCGACTTA
    CCGCAAGAGGTCGGAGAATATATTCCGTTTACTGCTGAAACACGTATGAGCGGTGTGAAA
    TTTACGACACGTGAAGTATATAAAGGTGCACCGAATAGTATGGTTAAGCGTGTGAAAGAA
    GCAGGGGGACATATTCCAGTTGATTTAGACGCTCTTGTCAAAGGGGTGTCTAAAAAAGGT
    GGCACACCGCTGGTTGTGCTTGAAGATAATGAGATTTTAGGTGTTATTTATTTGAAAGAT
    GTCATTAAAGATGGACTCGTAGAACGTTTCCGTGAATTACGTGAGATGGGGATTGAAACG
    GTGATGTGTACAGGAGATAACGAATTGACAGCTGCGACAATAGCGAAAGAAGCGGGTGTG
    GATCGCTTTGTGGCAGAGTGTAAACCTGAAGATAAAATCAATGTGATTAGAGAAGAACAA
    GCGAAAGGTCATATTGTTGCGATGACGGGTGACGGTACGAATGACGCGCCAGCTTTAGCA
    GAAGCTAATGTAGGTTTGGCAATGAACTCAGGAACCATGAGTGCCAAAGAAGCGGCGAAT
    TTAATTGATTTAGATTCTAATCCAACCAAACTGATGGAAGTCGTTCTAATTGGGAAACAA
    TTATTAATGACACGTGGCTCACTCACTACATTTAGTATTGCGAATGACATTGCGAAATAC
    TTTGCGATTTTACCAGCCATGTTTATGGCGGCTATGCCTGCGATGAATCATTTGAATATT
    ATGCATCTGCATTCACCTGAATCAGCAGTATTATCTGCGTTAATCTTTAATGCGTTGATT
    ATTGTATTATTGATTCCGATTGCGATGAAAGGCGTGAAATTTAAAGGTGCCTCAACGCAA
    ACCATATTGATGAAAAATATGTTAGTTTACGGCTTAGGCGGTATGATCGTGCCATTTATC
    GGCATTAAGCTCATTGATCTCATCATCCAACTCTTTGTCTAA
    60
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    1320
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    1560
    1620
    1680
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    1920
    1980
    2022

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS00495 [old locus tag: SA0070 ]
  • symbol: SA_RS00495
  • description: potassium-transporting ATPase subunit B
  • length: 673
  • theoretical pI: 6.05928
  • theoretical MW: 72984.3
  • GRAVY: 0.306241

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPase ATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, B subunit (TIGR01497; EC 3.6.3.12; HMM-score: 1026.8)
    and 19 more
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 307.4)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 274)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 233.7)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 219.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 219.4)
    plasma-membrane proton-efflux P-type ATPase (TIGR01647; EC 3.6.3.6; HMM-score: 180.1)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 128.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds calcium-translocating P-type ATPase, SERCA-type (TIGR01116; EC 3.6.3.8; HMM-score: 112.1)
    calcium-transporting P-type ATPase, PMR1-type (TIGR01522; EC 3.6.3.8; HMM-score: 106.5)
    P-type ATPase of unknown pump specificity (type V) (TIGR01657; HMM-score: 105.5)
    Metabolism Energy metabolism ATP-proton motive force interconversion Na,H/K antiporter P-type ATPase, alpha subunit (TIGR01106; EC 3.6.3.-; HMM-score: 97.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium-translocating P-type ATPase (TIGR01524; EC 3.6.3.2; HMM-score: 93)
    potassium/sodium efflux P-type ATPase, fungal-type (TIGR01523; EC 3.6.3.-; HMM-score: 84.3)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 27.5)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 23.2)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 20.4)
    phospholipid-translocating P-type ATPase, flippase (TIGR01652; EC 3.6.3.1; HMM-score: 16)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 13.2)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 13.2)
  • TheSEED: see SA0070
  • PFAM:
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 120.3)
    no clan defined E1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 119.8)
    and 3 more
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 27.9)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 21.3)
    P-loop_NTPase (CL0023) Herpes_TK; Thymidine kinase from herpesvirus (PF00693; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000993
    • TAT(Tat/SPI): 0.000069
    • LIPO(Sec/SPII): 0.000404
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAETTKIFESHLVKQALKDSVLKLYPVYMIKNPIMFVVEVGMLLALGLTIYPDLFHQESVSRLYVFSIFIILLLTLVFANFSEALAEGRGKAQANALRQTQTEMKARRIKQDGSYEMIDASDLKKGHIVRVATGEQIPNDGKVIKGLATVDESAITGESAPVIKESGGDFDNVIGGTSVASDWLEVEITSEPGHSFLDKMIGLVEGATRKKTPNEIALFTLLMTLTIIFLVVILTMYPLAKFLNFNLSIAMLIALAVCLIPTTIGGLLSAIGIAGMDRVTQFNILAKSGRSVETCGDVNVLILDKTGTITYGNRMADAFIPVKSSSFERLVKAAYESSIADDTPEGRSIVKLAYKQHIDLPQEVGEYIPFTAETRMSGVKFTTREVYKGAPNSMVKRVKEAGGHIPVDLDALVKGVSKKGGTPLVVLEDNEILGVIYLKDVIKDGLVERFRELREMGIETVMCTGDNELTAATIAKEAGVDRFVAECKPEDKINVIREEQAKGHIVAMTGDGTNDAPALAEANVGLAMNSGTMSAKEAANLIDLDSNPTKLMEVVLIGKQLLMTRGSLTTFSIANDIAKYFAILPAMFMAAMPAMNHLNIMHLHSPESAVLSALIFNALIIVLLIPIAMKGVKFKGASTQTILMKNMLVYGLGGMIVPFIGIKLIDLIIQLFV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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