From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00720 [old locus tag: SA0113 ]
  • pan locus tag?: SAUPAN000915000
  • symbol: SA_RS00720
  • pan gene symbol?: sbnB
  • synonym:
  • product: 2,3-diaminopropionate biosynthesis protein SbnB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00720 [old locus tag: SA0113 ]
  • symbol: SA_RS00720
  • product: 2,3-diaminopropionate biosynthesis protein SbnB
  • replicon: chromosome
  • strand: +
  • coordinates: 129749..130759
  • length: 1011
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (129749..130759) NCBI
  • BioCyc: SA_RS00720 BioCyc
  • MicrobesOnline: see SA0113

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATAGAGAGATGTTGTATTTAAATAGATCAGATATTGAACAAGCGGGAGGTAATCAT
    TCACAAGTTTATGTGGACGCATTAACAGAAGCATTAACAGCCCATGCGCACAATGATTTT
    GTACAACCGCTTAAGCCGTATTTAAGACAGGATCCTGAAAATGGACACATCGCAGATCGA
    ATTATTGCAATGCCAAGTCATATCGGTGGTGAACACGCAATTTCAGGTATTAAGTGGATA
    GGTAGTAAGCACGACAATCCATCGAAACGTAATATGGAGCGTGCAAGTGGTGTCATTATT
    TTGAATGATCCAGAAACGAATTATCCAATTGCAGTTATGGAAGCAAGTTTAATTAGTAGT
    ATGCGTACTGCAGCAGTTTCAGTGATTGCAGCTAAGCATTTGGCTAAAAAAGGATTTAAA
    GACTTAACAATCATTGGTTGCGGGCTAATCGGAGACAAGCAATTACAAAGTATGTTAGAG
    CAATTCGATCATATTAAACGCGTGTTTGTTTACGATCAATTCTCTGAAGCATGTGCACGC
    TTTGTTGATAGATGGCAACAACAGCGTCCGGAAATTAATTTTATTGCGACAGAAAATGCT
    AAAGAAGCAGTATCAAATGGTGAAGTAGTCATTACATGTACCGTAACGGATCAACCATAC
    ATTGAATATGATTGGTTACAAAAGGGTGCATTTATTAGCAACATTTCTATCATGGATGTG
    CATAAAGAAGTCTTTATTAAAGCTGACAAAGTCGTAGTAGATGACTGGTCACAATGTAAT
    CGAGAAAAGAAAACTATTAACCAATTGGTGTTAGAAGGTAAATTCAGCAAAGAAGCACTT
    CATGCTGAACTAGGGCAACTTGTGACAGGTGACATACCAGGACGTGAAGACGATGATGAA
    ATCATATTACTTAATCCGATGGGTATGGCTATCGAAGATATTTCAAGTGCTTATTTTATT
    TATCAACAGGCACAACAACAAAATATTGGGACAACATTGAACCTATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS00720 [old locus tag: SA0113 ]
  • symbol: SA_RS00720
  • description: 2,3-diaminopropionate biosynthesis protein SbnB
  • length: 336
  • theoretical pI: 5.10763
  • theoretical MW: 37747.6
  • GRAVY: -0.29256

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 420.7)
    and 3 more
    alanine dehydrogenase (TIGR02371; EC 1.4.1.1; HMM-score: 189.9)
    ectoine utilization protein EutC (TIGR02992; HMM-score: 100.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 16)
  • TheSEED: see SA0113
  • PFAM:
    NADP_Rossmann (CL0063) OCD_Mu_crystall; Ornithine cyclodeaminase/mu-crystallin family (PF02423; HMM-score: 218.4)
    and 5 more
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 24)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 16.2)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14.1)
    Ig (CL0011) Ig_3; Immunoglobulin domain (PF13927; HMM-score: 14)
    NADP_Rossmann (CL0063) THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010764
    • TAT(Tat/SPI): 0.002157
    • LIPO(Sec/SPII): 0.001169
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPLKPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILNDPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLEQFDHIKRVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQPYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFSKEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLNLY

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]