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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01330 [old locus tag: SA0227 ]
  • pan locus tag?: SAUPAN001096000
  • symbol: SA_RS01330
  • pan gene symbol?: fadX
  • synonym:
  • product: acetyl-CoA/acetoacetyl-CoA transferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01330 [old locus tag: SA0227 ]
  • symbol: SA_RS01330
  • product: acetyl-CoA/acetoacetyl-CoA transferase
  • replicon: chromosome
  • strand: -
  • coordinates: 273754..275331
  • length: 1578
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (273754..275331) NCBI
  • BioCyc: SA_RS01330 BioCyc
  • MicrobesOnline: see SA0227

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
    GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
    GCTGTGTTAGCGCAACATGACACATATCATACGCCCAAAGATTTAACGTTTATATTAGCG
    AATGATATCCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
    ATTAAACGTGTCATTATGAGCATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
    AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
    AGTAATCAATTATTACCTGGAGTTATTACTAAAATCGGATTAAACACAGCTGTTGATCCT
    AGATACGGTGGCGGTAAAGTAAATACACGAACAACTGATGATTTAGTTTCATTAGTAACC
    ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
    GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
    TGTTATCATGTCGCATTAAACGCGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
    GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
    GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAGAGTCATTAT
    TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
    AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
    AGCATCGGTTATGGCATCAATAATGAACTCTCTAATTTATTGCACGAAGAATGTGTTGAA
    CATGATGTGCAACCGATTCTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
    CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
    ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
    AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGTGGCTTTATAGACATT
    ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
    TGTCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
    GTGAGCCATATCGACTTTAATGCACAATATTCACAATTACTCGAGCAAGAAGTCTATTTT
    GTTACTGATCGTGCAGTATTCGAGCTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
    CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
    GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
    TCAATTCATAAAGTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS01330 [old locus tag: SA0227 ]
  • symbol: SA_RS01330
  • description: acetyl-CoA/acetoacetyl-CoA transferase
  • length: 525
  • theoretical pI: 5.62104
  • theoretical MW: 58902.3
  • GRAVY: -0.15581

Function[edit | edit source]

  • TIGRFAM:
    3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 41.5)
    and 3 more
    3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.8)
    Metabolism Transport and binding proteins Cations and iron carrying compounds malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.5)
    Metabolism Energy metabolism Other malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.5)
  • TheSEED: see SA0227
  • PFAM:
    ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 71.7)
    and 3 more
    Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 22.4)
    AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 19.1)
    no clan defined Curlin_rpt; Curlin associated repeat (PF07012; HMM-score: 11.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012359
    • TAT(Tat/SPI): 0.002349
    • LIPO(Sec/SPII): 0.000585
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQLLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]