PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02470 [old locus tag: SA0434 ]
• pan locus tag^?: SAUPAN002192000
• symbol: SA_RS02470
• pan gene symbol^?: treR
• synonym:
• product: trehalose operon repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02470 [old locus tag: SA0434 ]
• symbol: SA_RS02470
• product: trehalose operon repressor
• replicon: chromosome
• strand: +
• coordinates: 500630..501358
• length: 729
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (500630..501358) NCBI
• BioCyc: SA_RS02470 BioCyc
• MicrobesOnline: see SA0434

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCGAAACAAAAAAAGTTTATGAAGATTTATGAGGCGTTGAAAGAAGATATATTAAAC
  GGGCAGATTCAATATGGTGAACAAATTCCGTCTGAACATGATTTGGTGCAATTGTACCAG
  TCATCTCGAGAGACCGTGCGTAAGGCATTAGATTTGTTGGCATTAGACGGCATGATTCAA
  AAGATTCATGGTAAAGGGTCACTTGTCATTTATCAGGAGGTTACAGAGTTTCCATTTTCT
  GAACTTGTTAGTTTTAAAGAAATGCAAGAAGAAATGGGCGTCGCATATTTAACTGAAGTT
  GTTGTGAATGAGGTTGTTGAAGCGCATGAAGTTCCAGAAGTTCAACATGCTTTAAACATC
  AATTCTAGTGAATCGCTCATTCATATTGTTAGAACTCGTCGGCTTAACCAACATGTGAAG
  ATTGTTGATGAAGATTATTTTCTAAAGTCGATTGTTTCAGATATAGGTAATGATGTTGCG
  AGTGATTCTATTTATGATTATTTGGAAAAGGTATTAAATCTTAATATTAGTTATTCAAGT
  AAGTCTATTACTTTTGAACCGTTTGATGAACAAGCATATCAATTGTTTGGTGATGTATCG
  GTGGCTTATTCAGCAACAGTTCGAAGTATTGTGTATTTAGAAAATACAATGCCGTTTCAA
  TATAATATTTCAAAACATCTTGCAAATGAATTTAAATTTAACGACTTCTCAAGACGTCGT
  ATAAAGTAA
  60
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  729
  

⊟Protein

⊟General

• locus tag: SA_RS02470 [old locus tag: SA0434 ]
• symbol: SA_RS02470
• description: trehalose operon repressor
• length: 242
• theoretical pI: 4.92255
• theoretical MW: 27962.5
• GRAVY: -0.266116

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 229.5)
  and 10 more
  Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 72.4)
  Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 72.4)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 62)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 62)
  Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 57.1)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 23.3)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 23.3)
  Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 23.3)
  phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 17.4)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replisome organizer, putative, N-terminal region (TIGR01714; HMM-score: 14.7)
• TheSEED: see SA0434
• PFAM:
  UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 91.5)
  and 4 more
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 60)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 20.9)
  HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 14.3)
  Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by TreR*, TF important in Trehalose utilization: see SA0434

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002243
  • TAT(Tat/SPI): 0.000124
  • LIPO(Sec/SPII): 0.000191
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447041419 NCBI
• RefSeq: WP_001118675 NCBI
• UniProt: see SA0434

⊟Protein sequence

• MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRETVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVVNEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVASDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMPFQYNISKHLANEFKFNDFSRRRIK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, TreR* see SA0434

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications